Why does increasing extracellular pH increase the proton motive force in bacteria?

I thought increasing proton concentration (lowering pH) would increase the proton motive force, but I'm reading that the opposite occurs. Trying to understand why increasing pH increases...

11 December 2019 4,549 3 View

Can anyone help me predict if the fluorescence spectrum of NBD-CO-Hz will change in this reaction?

I am reacting NBD-CO-Hz with oxalate. When reacted (condensed) with a single carboxylic acid-containing molecule the fluorescence spectrum of NBD-CO-Hz does not normally change much. It is...

08 September 2019 1,380 1 View

Why is PDAM Giving me this Result on UPLC?

I have purchased PDAM from two different suppliers, and am getting the same problem both times. This chemical should be non-fluorescent until reacted with a carboxylic acid, but when we run the...

06 July 2019 6,454 3 View

Why would TCEP in water produce fluorescent products with ABD-F?

I am derivatizing GSH with ABD-F to make the glutathione fluorescent. I am using TCEP to reduce GSSG. When I run a blank control with just water (ultrapure) instead of GSSG the TCEP appears to...

31 December 2018 2,375 1 View

PDAM (1-Pyrenyldiazomethane) fluorescence derivatization of oxalate?

Hello, I have a question about PDAM that I hope someone can help me with. I would like to measure oxalate (a dicarboxylic acid) in aqueous samples, and I need to find a suitable fluorescent...

31 December 2018 8,893 4 View

Incubating cells in 50 mM bicarbonate, pH 7.4, why does intracellular pH go down?

I am performing an experiment in CHO cells with or without a chloride/bicarbonate exchanger protein expressed in the membrane. When I replace normal buffer containing 130 mM chloride (pH 7.4)...

05 June 2018 524 2 View

How long can CHO cells function without magnesium?

I am studying transport of something that can bind to and be inhibited by magnesium, so I am excluding magnesium from the transport buffer. I find that after washing the cells and incubating...

03 April 2018 5,509 3 View

What is the best way to irreversibly protect or modify the nitrogens in urate to make them tertiary? I need to accomplish this in aqueous?

What is the best way to irreversibly protect or modify the nitrogens in urate to make them tertiary? I need to accomplish this in aqueous conditions. I am trying to modify them to avoid a...

02 March 2018 222 0 View

Does phenyl isothiocyanate react with the amines in uric acid?

PITC reacts with ammonia, primary, and secondary amines. Therefore, it should react with uric acid, correct?

10 November 2017 6,603 0 View

In a suspension of Ca oxalate is it possible to pull the Ca from oxalate and force it to bind to carbonate? If I supply abundant Na bicarbonate?

04 May 2017 2,739 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View