14 Questions 46 Answers 0 Followers
Questions related from Adam L Vanwert
I thought increasing proton concentration (lowering pH) would increase the proton motive force, but I'm reading that the opposite occurs. Trying to understand why increasing pH increases...
12 December 2019 4,675 3 View
I am reacting NBD-CO-Hz with oxalate. When reacted (condensed) with a single carboxylic acid-containing molecule the fluorescence spectrum of NBD-CO-Hz does not normally change much. It is...
09 September 2019 1,472 1 View
I have purchased PDAM from two different suppliers, and am getting the same problem both times. This chemical should be non-fluorescent until reacted with a carboxylic acid, but when we run the...
07 July 2019 6,541 3 View
Hello, I have a question about PDAM that I hope someone can help me with. I would like to measure oxalate (a dicarboxylic acid) in aqueous samples, and I need to find a suitable fluorescent...
01 January 2019 8,976 4 View
I am derivatizing GSH with ABD-F to make the glutathione fluorescent. I am using TCEP to reduce GSSG. When I run a blank control with just water (ultrapure) instead of GSSG the TCEP appears to...
01 January 2019 2,469 1 View
CHO cells are reportedly of epithelial origin. I have a transporter stably transfected in the CHO cells that is normally apical in the colon. Will it be exclusively expressed on the top aspect...
07 July 2018 407 2 View
I am performing an experiment in CHO cells with or without a chloride/bicarbonate exchanger protein expressed in the membrane. When I replace normal buffer containing 130 mM chloride (pH 7.4)...
06 June 2018 602 2 View
I am studying transport of something that can bind to and be inhibited by magnesium, so I am excluding magnesium from the transport buffer. I find that after washing the cells and incubating...
04 April 2018 5,604 3 View
What is the best way to irreversibly protect or modify the nitrogens in urate to make them tertiary? I need to accomplish this in aqueous conditions. I am trying to modify them to avoid a...
03 March 2018 311 0 View
PITC reacts with ammonia, primary, and secondary amines. Therefore, it should react with uric acid, correct?
11 November 2017 6,708 0 View
Calcium carbonate and calcium oxalate are both highly insoluble in water. Is it possible to shift the calcium from oxalate to carbonate if enough carbonate is supplied (in vitro).
05 May 2017 2,823 2 View
Why can't I use sulfuric acid, hydrochloric acid, etc.?
10 October 2016 1,981 5 View
I've performed a reaction several times over the past few months, and this is the first time I experienced a new problem. Normally I add diethylenetriamine to acetonitrile to serve as a...
10 October 2016 263 5 View
I found this reaction in an article, but I'm wondering if the center carbon in the triazacyclononane will truly be removed spontaneously in the reaction. Also, the solvent used in the reaction...
09 September 2015 3,430 0 View
