Why does increasing extracellular pH increase the proton motive force in bacteria?

I thought increasing proton concentration (lowering pH) would increase the proton motive force, but I'm reading that the opposite occurs. Trying to understand why increasing pH increases...

11 December 2019 4,549 3 View

Can anyone help me predict if the fluorescence spectrum of NBD-CO-Hz will change in this reaction?

I am reacting NBD-CO-Hz with oxalate. When reacted (condensed) with a single carboxylic acid-containing molecule the fluorescence spectrum of NBD-CO-Hz does not normally change much. It is...

08 September 2019 1,380 1 View

Why is PDAM Giving me this Result on UPLC?

I have purchased PDAM from two different suppliers, and am getting the same problem both times. This chemical should be non-fluorescent until reacted with a carboxylic acid, but when we run the...

06 July 2019 6,454 3 View

Why would TCEP in water produce fluorescent products with ABD-F?

I am derivatizing GSH with ABD-F to make the glutathione fluorescent. I am using TCEP to reduce GSSG. When I run a blank control with just water (ultrapure) instead of GSSG the TCEP appears to...

31 December 2018 2,375 1 View

PDAM (1-Pyrenyldiazomethane) fluorescence derivatization of oxalate?

Hello, I have a question about PDAM that I hope someone can help me with. I would like to measure oxalate (a dicarboxylic acid) in aqueous samples, and I need to find a suitable fluorescent...

31 December 2018 8,893 4 View

Do CHO cells polarize on Transwell inserts?

CHO cells are reportedly of epithelial origin. I have a transporter stably transfected in the CHO cells that is normally apical in the colon. Will it be exclusively expressed on the top aspect...

06 July 2018 328 2 View

Incubating cells in 50 mM bicarbonate, pH 7.4, why does intracellular pH go down?

I am performing an experiment in CHO cells with or without a chloride/bicarbonate exchanger protein expressed in the membrane. When I replace normal buffer containing 130 mM chloride (pH 7.4)...

05 June 2018 524 2 View

How long can CHO cells function without magnesium?

I am studying transport of something that can bind to and be inhibited by magnesium, so I am excluding magnesium from the transport buffer. I find that after washing the cells and incubating...

03 April 2018 5,509 3 View

Does phenyl isothiocyanate react with the amines in uric acid?

PITC reacts with ammonia, primary, and secondary amines. Therefore, it should react with uric acid, correct?

10 November 2017 6,603 0 View

In a suspension of Ca oxalate is it possible to pull the Ca from oxalate and force it to bind to carbonate? If I supply abundant Na bicarbonate?

04 May 2017 2,739 2 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

Gas sensor response and recovery time independant of flow rate and chamber volume?

I am trying to obtain the response and recovery times for my gas sensor device. It appears the the response/recovery times are primarily dependant on the flow rate of the gas I inject into the...

26 February 2021 1,511 3 View

Why do a weight decrease for thermal degradation in nitrogen gas condition?

Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...

26 February 2021 1,100 5 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

How to delaminate copper in water-based anodes?

Hello everyone, I'm looking for a way to delaminate copper current collector of water-based anodes in Li-ion batteries without damaging the graphite film. I tried different materials such as HNO3...

24 February 2021 3,785 3 View

Open Source IoT Simulators supported by Machine Learning?

Dear Research Gaters, Let me first add context to the question. I am looking to simulate the waste management system and resources of a city. The hardware devices I need to simulate include,...

21 February 2021 2,333 4 View

What type of pressure sensor are usually used in microfluidics?

My team is designing a dual channel microfluidic chip that runs air and water. I think we've decided on the NE1000 syringe pump for it's versatility and feedback control. We will be ordering the...

18 February 2021 9,928 2 View

Can COVID-19 vaccines protect against the emerging strains of SARS-CoV-2?

Can COVID-19 vaccines protect against the emerging strains of SARS-CoV-2?

18 February 2021 8,221 8 View

How to realize refractive index values of 1 to 1.1?

Hello researchers! While studying the literature on refractive index sensors, most of the articles simulate their devices with a refractive index ranging from 1 to 1.1, with small steps in...

17 February 2021 1,578 4 View