What is the best way to irreversibly protect or modify the nitrogens in urate to make them tertiary? I need to accomplish this in aqueous conditions. I am trying to modify them to avoid a reaction with a copper compound that I'm using as a sensor.
I thought increasing proton concentration (lowering pH) would increase the proton motive force, but I'm reading that the opposite occurs. Trying to understand why increasing pH increases...
11 December 2019 4,719 3 View
I am reacting NBD-CO-Hz with oxalate. When reacted (condensed) with a single carboxylic acid-containing molecule the fluorescence spectrum of NBD-CO-Hz does not normally change much. It is...
08 September 2019 1,520 1 View
I have purchased PDAM from two different suppliers, and am getting the same problem both times. This chemical should be non-fluorescent until reacted with a carboxylic acid, but when we run the...
06 July 2019 6,594 3 View
I am derivatizing GSH with ABD-F to make the glutathione fluorescent. I am using TCEP to reduce GSSG. When I run a blank control with just water (ultrapure) instead of GSSG the TCEP appears to...
31 December 2018 2,537 1 View
Hello, I have a question about PDAM that I hope someone can help me with. I would like to measure oxalate (a dicarboxylic acid) in aqueous samples, and I need to find a suitable fluorescent...
31 December 2018 9,031 4 View
CHO cells are reportedly of epithelial origin. I have a transporter stably transfected in the CHO cells that is normally apical in the colon. Will it be exclusively expressed on the top aspect...
06 July 2018 465 2 View
I am performing an experiment in CHO cells with or without a chloride/bicarbonate exchanger protein expressed in the membrane. When I replace normal buffer containing 130 mM chloride (pH 7.4)...
05 June 2018 652 2 View
I am studying transport of something that can bind to and be inhibited by magnesium, so I am excluding magnesium from the transport buffer. I find that after washing the cells and incubating...
03 April 2018 5,657 3 View
PITC reacts with ammonia, primary, and secondary amines. Therefore, it should react with uric acid, correct?
10 November 2017 6,761 0 View
Calcium carbonate and calcium oxalate are both highly insoluble in water. Is it possible to shift the calcium from oxalate to carbonate if enough carbonate is supplied (in vitro).
04 May 2017 2,880 2 View
The paper in question is "Interpolation of Nitrogen Fertilizer Use in Canada from Fertilizer Use Surveys". This paper was very recently published by Agronomy (MDPI). Agronomy has, in the last day...
07 August 2024 9,934 3 View
My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...
07 August 2024 4,600 2 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View
Reference dose and Maximum acceptable concentrations HMs
03 August 2024 8,230 4 View
What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?
01 August 2024 1,722 1 View
I've attempted to use this method to synthesize copper nanoparticles. Copper nanoparticles can be synthesised using a variety of precursor materials. CuSO4, distilled water, NaOH, and EDTA are...
28 July 2024 8,027 3 View
need of sensor details or sensor development for this question.
28 July 2024 4,539 3 View
What has been the social protection outreach like in Yobe state. Which areas are challenging for the government departments and what capacity building would be most effective? Comparison of the...
21 July 2024 9,887 1 View
I try to understand the ESP mechanism working. What kind of sensors it has, Is it necessary to modify or boost its mechanical ?
15 July 2024 4,772 1 View
Our lab has some radioactive cell samples that need to be kept frozen for at least 30 days before flow analysis. For some reason, the samples stored in liquid nitrogen did not perform as well as...
15 July 2024 9,975 1 View