Why does increasing extracellular pH increase the proton motive force in bacteria?

I thought increasing proton concentration (lowering pH) would increase the proton motive force, but I'm reading that the opposite occurs. Trying to understand why increasing pH increases...

11 December 2019 4,549 3 View

Can anyone help me predict if the fluorescence spectrum of NBD-CO-Hz will change in this reaction?

I am reacting NBD-CO-Hz with oxalate. When reacted (condensed) with a single carboxylic acid-containing molecule the fluorescence spectrum of NBD-CO-Hz does not normally change much. It is...

08 September 2019 1,380 1 View

Why is PDAM Giving me this Result on UPLC?

I have purchased PDAM from two different suppliers, and am getting the same problem both times. This chemical should be non-fluorescent until reacted with a carboxylic acid, but when we run the...

06 July 2019 6,454 3 View

Why would TCEP in water produce fluorescent products with ABD-F?

I am derivatizing GSH with ABD-F to make the glutathione fluorescent. I am using TCEP to reduce GSSG. When I run a blank control with just water (ultrapure) instead of GSSG the TCEP appears to...

31 December 2018 2,375 1 View

PDAM (1-Pyrenyldiazomethane) fluorescence derivatization of oxalate?

Hello, I have a question about PDAM that I hope someone can help me with. I would like to measure oxalate (a dicarboxylic acid) in aqueous samples, and I need to find a suitable fluorescent...

31 December 2018 8,893 4 View

Do CHO cells polarize on Transwell inserts?

CHO cells are reportedly of epithelial origin. I have a transporter stably transfected in the CHO cells that is normally apical in the colon. Will it be exclusively expressed on the top aspect...

06 July 2018 328 2 View

Incubating cells in 50 mM bicarbonate, pH 7.4, why does intracellular pH go down?

I am performing an experiment in CHO cells with or without a chloride/bicarbonate exchanger protein expressed in the membrane. When I replace normal buffer containing 130 mM chloride (pH 7.4)...

05 June 2018 524 2 View

How long can CHO cells function without magnesium?

I am studying transport of something that can bind to and be inhibited by magnesium, so I am excluding magnesium from the transport buffer. I find that after washing the cells and incubating...

03 April 2018 5,509 3 View

What is the best way to irreversibly protect or modify the nitrogens in urate to make them tertiary? I need to accomplish this in aqueous?

What is the best way to irreversibly protect or modify the nitrogens in urate to make them tertiary? I need to accomplish this in aqueous conditions. I am trying to modify them to avoid a...

02 March 2018 222 0 View

Does phenyl isothiocyanate react with the amines in uric acid?

PITC reacts with ammonia, primary, and secondary amines. Therefore, it should react with uric acid, correct?

10 November 2017 6,603 0 View

How can we obtain a modification factor for creep parameters?

I want to obtain standard creep parameters of salt rock by creep parameters of rectangular samples. Is there a modification factor to convert creep parameters of rectangular (prismatic) rock salt...

01 March 2021 7,131 1 View

Anyone found any research papers on solar furnace design calculations (for solar concentration)?

I am thinking about working on solar furnace with heliostats that concentrate sunlight and heat molten salt, but currently no luck with research. Not a lot of papers based on the subject. (check...

01 March 2021 5,023 5 View

Which transition metal salt precursor is best for doping? Why?

I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...

27 February 2021 4,285 3 View

What are the saturated salt solutions that can give specific relative humidity of 35%, 65% and 95% for conditioning wood specimens?

Due to the unavailability of climate rooms, I want to carry out an experiment that require the use of certain saturated salt solutions especially those that can condition the wood specimen to 35%,...

25 February 2021 8,139 1 View

It is necessary to reflux the Schiff base and metal salts to obtain metal complex??

24 February 2021 9,166 3 View

How can I remove deep eutectic solvents from the medium?

Dear researchers, How can I remove deep eutectic solvents from the medium? You know, generally, deep eutectic solvents consist of ammonium salts and an acid group. Is there any alternative method...

22 February 2021 3,275 3 View

Physical-chemical formation of stalactites/stalagmites. what promotes the formation/growth of these structures?

I know, or think I do, the basic idea behind these natural structures, as from what I know they form due to water dripping from the top leaving a salt deposit behind. But started to wonder about...

22 February 2021 1,400 4 View

How to prepare electrolyte of NaClO4 salt for Na-ion battery? Any problem with this salt?

Hello, I am trying to make anode for Na-ion batteries. I have no experience with preparation of the electrolyte for any batteries. NaClO4 salt are available and I am planning to use EC:PC as...

21 February 2021 8,132 1 View

When I reacted Bidentate NS Schiff Base with metal salt (M-Cl2) in +2 oxidation state in proportion of (2:1) , getting sticky product ,, why?

When I reacted Bidentate NS Schiff Base with metal salt (M-Cl2) metal chlorides in +2 oxidation state in proportion of (2:1 Schiff base &metal salt) , i was get sticky material Why ? ---~...

21 February 2021 7,970 5 View

What is the nutrient requirements for E.coli BL21 CodonPlu RIPL strain growing in M9 medium?

Hello. I want to isotopically label the my protein, and I express it in BL21 RIPL using M9 minimal media. It grows extremely slow. I inoculate the colony from a fresh M9 plate into 5ml M9 medium...

17 February 2021 2,852 2 View