PITC reacts with ammonia, primary, and secondary amines. Therefore, it should react with uric acid, correct?
I thought increasing proton concentration (lowering pH) would increase the proton motive force, but I'm reading that the opposite occurs. Trying to understand why increasing pH increases...
11 December 2019 4,719 3 View
I am reacting NBD-CO-Hz with oxalate. When reacted (condensed) with a single carboxylic acid-containing molecule the fluorescence spectrum of NBD-CO-Hz does not normally change much. It is...
08 September 2019 1,520 1 View
I have purchased PDAM from two different suppliers, and am getting the same problem both times. This chemical should be non-fluorescent until reacted with a carboxylic acid, but when we run the...
06 July 2019 6,594 3 View
I am derivatizing GSH with ABD-F to make the glutathione fluorescent. I am using TCEP to reduce GSSG. When I run a blank control with just water (ultrapure) instead of GSSG the TCEP appears to...
31 December 2018 2,537 1 View
Hello, I have a question about PDAM that I hope someone can help me with. I would like to measure oxalate (a dicarboxylic acid) in aqueous samples, and I need to find a suitable fluorescent...
31 December 2018 9,031 4 View
CHO cells are reportedly of epithelial origin. I have a transporter stably transfected in the CHO cells that is normally apical in the colon. Will it be exclusively expressed on the top aspect...
06 July 2018 465 2 View
I am performing an experiment in CHO cells with or without a chloride/bicarbonate exchanger protein expressed in the membrane. When I replace normal buffer containing 130 mM chloride (pH 7.4)...
05 June 2018 652 2 View
I am studying transport of something that can bind to and be inhibited by magnesium, so I am excluding magnesium from the transport buffer. I find that after washing the cells and incubating...
03 April 2018 5,657 3 View
What is the best way to irreversibly protect or modify the nitrogens in urate to make them tertiary? I need to accomplish this in aqueous conditions. I am trying to modify them to avoid a...
02 March 2018 364 0 View
Calcium carbonate and calcium oxalate are both highly insoluble in water. Is it possible to shift the calcium from oxalate to carbonate if enough carbonate is supplied (in vitro).
04 May 2017 2,880 2 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
The first pdf file I uploaded had an error. So I uploaded an updated, corrected pdf of that paper with a different pdf name. I dpon't want the old copy to be download or read.
07 August 2024 9,508 1 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?
02 August 2024 7,186 6 View
I'm trying to calculate the tolerance factors of the hydride perovskite and the value calculated by me and the reported values are contradictory. Need more clarity in using the correct ionic radii...
01 August 2024 2,614 1 View
A typical reforming section in a large-scale Haber-Bosch process (Figure 1) comprises two reactors. A mixture of methane (CH4) and steam (H2O) is fed into a primary reformer to produce syngas via...
01 August 2024 7,051 5 View
How to change the displayed full article text to its corrected version? In the file on the page of the journal where I published the article, there was an error in the text, the table is...
30 July 2024 3,229 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
The entropy measured of molecular graphs plays a crucial rule. The network structures in some cases are very lengthy calculations to handle. Some author avoid to construct table where as most...
30 July 2024 3,126 0 View
I am writing to request a correction regarding the affiliation of one of my co-authors, Donald Ho. Currently, the author's profile incorrectly lists an affiliation with The Hong Kong Polytechnic...
28 July 2024 6,824 0 View