Do we use inactivated FBS for all cell culture expansion?
Or is it based on the end point eg: immunomodulatory studies
Since the primary purpose of heat inactivation is denaturation of complement, it is not necessary to heat inactivate in a great many non-immune system cell or tissue culture applications. It can interfere with some growth factor activity.
https://www.researchgate.net/file.PostFileLoader.html?id=530e06f3d4c11817068b45a8&assetKey=AS%3A272439907422216%401441966143594
William Richard Webb Thank you, any particular reason why we do need to add inactivated FBS for pellet cultures?
Ellen A G Chernoff Aman Sharma
Thank you for the technical information sheet
Very helpful.
so, heat inactivation, when performed properly, offers little or no advantages for cell growth and in a few cell lines may cause a decrease in cell growth.
Warming to 37°C prior to use, itself would inactivate heat-labile complement factors.
yes, its rather simple really you want to standardise the differentiation in regards ITS-X, vitamin C, Growth factor and DEX (if you use dex). Normal FBS (if not inactivated) will contain growth factors that can influence outcome.
IN Our lab, we only use Human cells and we have now switched from using FBS for expansion and our differentiation media contains NO FBS we use a substitute that is defined. Having defined differentiation media which is standardised removes external influences. SO its down to components of differentiation media used in 3D culture and the cells alone (and not what might be in the FBS)
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