Elizabeth Vinod

5 Questions 26 Answers 0 Followers

Questions related from Elizabeth Vinod

For growing human dendritic cells and PBMCs ,can we use IL-4 and GMCSF expressed in E.coli instead of human cell expressed?

And for co-culture studies would CD4+ tcells with our cell of interest be ideal to check for PGE2, IFN gamma and NO expression or PBMC with the cells ? Human dendritic cells as positive markers...

08 November 2016 9,788 8 View

Processed a hyaline cartilage tissue for IHC- DAPI stained area around it but not the nucleus- can somebody tell me why and what needs to be done??

formalin fixed- pll coated- primary followed by secondary then DAPI- permeabilization was done with triton X

14 April 2016 2,717 2 View

Low sample size : statistical analysis

1. What statistical analysis can we use for a low sample size (n=3): between 2 groups and between more than 2 groups 2. If we take two samples and run them in technical replicates of say 6, can my...

01 January 1970 2,566 7 View

Inactivated Fetal bovine serum for cell culture

Do we use inactivated FBS for all cell culture expansion? Or is it based on the end point eg: immunomodulatory studies

01 January 1970 9,191 7 View

Exosome characterization : CD9

Isolated exosomes displaying CD81 and CD63 positivity but absent for CD9. CD9 positive control shows no issues with the antibody. Anti-CD63 beads were used, and FACS gating was based on the...

01 January 1970 4,635 3 View