Could you share your protocol in coating Gold electrode by Fibronectin?

06 July 2014 587 1 View

How can I dilute Cisplatin?

I would like to test the response of cancer cells (MDA and MCF7) on Cisplatin. I bought a bottle (15g) in powder. Could you tell me how should I dilute it? Which concentration should I use? How...

05 June 2014 7,921 7 View

How do you make CO2 independent medium for breast cancer cell?

I am using MDA-MB-231 and MCF7 for doing experiment. We have CO2 independent medium. Could you tell me how should I add other components (FBS, Glutamin/max, Penicillin, Insulin..). If I need 1ml...

02 March 2014 9,953 2 View

How to modify gold electrode by nanowires?

Do you have an experience in modifying gold electrode by nanowire? How we can modify gold electrode by ZnO or Au nanowire? Which type of nanowire is better for cell-based sensors?

09 October 2013 2,592 2 View

How to coat graphene oxide or graphite oxide on a gold electrode?

I would like to modify our gold electrode by GO or r-GO. Does anyone have any experience?

09 October 2013 1,959 7 View

What are advantages in using graphene for cell-based sensor?

How we can modify gold electrode with graphene? What are advantages in using graphene based electrode for cell-based sensor?

08 September 2013 9,047 2 View

How can I measure multichannel with Solartron 1260.

I have electrode arrays and I would like to measure multichannel with SI 1260. Are you working with this machine? If you know, please help me.

07 August 2013 7,663 1 View

Does anyone use Solartron 1260 to measure impedance? Did you measure V1/V2?

I have three electrodes: WE, CE and RE. How can I connect with SI 1260 to measure V1/V2?

02 March 2013 5,059 2 View

What are the reasons leading to "Restricted diffusion" in electrochemistry? Which documents are best to understand this phenomenon?

My system consists of two gold electrodes inside a microchannel (300 micrometer width and 50 micrometer height) covered by a medium for cells. The surface of electrodes is modified with SAM and...

01 February 2013 2,545 0 View

How can I remove Thiol from a gold electrode?

I modified my gold electrode by 11-mercaptoundecanoic acid (11-MUA) in ethanol solution 1mM in 18h. I would like to clean and remove this SAM layer to reuse the chips.

31 December 2012 5,990 8 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View