Ceren Günes

6 Questions 24 Answers 0 Followers

Questions related from Ceren Günes

How can I sort human neural stem cells without killing them?

I tried to sort human neural stem cells (induced from hiPSC) and observed that almost all of the cells die after the sort, although they were alive before. I used 100um nozzle, and PBS-EB (EDTA...

01 January 2016 5,963 7 View

Fluorescent signal reduction after fixation/permeabilization?

I infected human neural stem cells with a RFP tagged viral construct, and wanted to sort infected and undifferentiated cells. For this I use two intracellular markers, Sox2 and Nestin. When I ran...

01 January 2016 6,664 7 View

How can I differentiate between endogenous vs. exogenous gene expression?

During somatic cell reprogramming to iPSC, cells get transiently transduced with reprogramming factors. When it comes to measuring the expression level of these factors, how could one...

12 December 2015 2,016 3 View

Any advice on a FACS analysis of iPSC?

I tried sorting human iPSC by flow cytometry, but lost a lot of cells during staining or transfer of cells from tubes to epis or so. Any suggestions to prevent this? I know that some people coat...

12 December 2015 8,813 4 View

Has anyone performed LTC-IC assay on human CD34+ HSC with hTERT immortalised mesencyhmal stem cell line, SCP-1?

I have been trying to keep CD34+ HSC undifferentiated in suspension culture, and compared some culture conditions with different cytokine combinations, etc. I also tried the co-culture on primary...

03 March 2015 4,128 1 View

Neurosphere like formation from human NSC?

I derived human neural stem/progenitor cells from iPSC and have been doing RNAi screen on the NSPC. Normally they are adherent cells and form a network like structure. But when I knock down this...

01 January 1970 7,889 5 View