Adil Karim

6 Questions 3 Answers 0 Followers

Questions related from Adil Karim

I want to see the interaction of STAT3 & NFkB after IL-6 treatment by Co-IP?

Dear All, I performed the Co-IP to see the binding and co-localization of STAT3: and NFkB p50 in Human PBMcs Co- immunoprecipitation were performed on PBMCs (4×106) from cells treated with and...

07 July 2019 5,937 4 View

Is it any compensation issue or flow antibody titration or something else?

I stained four tubes: Tube 1: Unstained Tube 2: Stained with only Total STAT3 labelled with PerCP cy 5.5. Tube 3: Stained with only P-STAT3 (Y705) labelled with Alexa flour 647.(APC Chanel...

09 September 2018 6,571 4 View

Amount of RNA later to preserve urine sample ?

I want to preserve urine samples. Can anyone suggest the volume of RNA later to be put in 6-7 ml urine sample ??

03 March 2018 4,796 2 View

RNA isolation kit from urine sample ?

Hiiii Can anyone advice regarding the best kit for RNA isolation from urine samples? I have used qiagen viral RNA kit but it gives vey high O.D ( ~ 3.1 ) I need purified RNA with good quality so...

03 March 2018 2,420 2 View

Is there any other marker or antibodies apart from IL-17A (BD Biosciencs ) for the detection of IL-17 cells by Flow cytometry?

Hiii, For the detection of Th17 cells, I am using IL-17A antibodies from BD Biosciences in the patients with Hyper IgE syndrome.But my results (percentage of IL-17 cells) drastically vary from...

01 January 2018 4,592 2 View

I want to see the RhoGTPase expression on VTA?

I want to see the RhoGTPase expression on VTA by the help of flowcytometry in mice model??

12 December 2017 2,033 1 View