Pranay Mandal

6 Questions 16 Answers 0 Followers

Questions related from Pranay Mandal

What is the difference between Bacterial DNA and eukaryotic Mitochondrial DNA?

If I get a piece of DNA, how can I tell if the DNA is from Mitochondria of Bacteria?

09 September 2019 7,138 6 View

Can Laminin be used twice for mice cortical neuronal culture?

I used laminin at a concentration of 10ug/ml for growing mice cortical neurons. Can I store it in -20 and re-use it?

10 October 2018 9,812 1 View

Does YeGFP expresses in mammalin cell lines like HEK?

I have cloned YeGFP in a mammalian expression vector, but I don't see any GFP expressionin HEK cells. Is YeGFP is supposed to fluoresce in mammalian cells?

10 October 2018 9,122 1 View

Is there any protocol for myelin coating?

I am extracting myelin fro rat brain following Norton and Poduslo, 1973 method, but I don't know how to use or process the myelin after that. Also, I couldn't find any protocol for plating myelin....

11 November 2017 946 3 View

What concentration of Laminin is ideal for neuronal culture?

What concentration of Laminin is ideal for neuronal culture? And should I dissolve it in water or PBS?

09 September 2016 7,680 11 View

Is there any different way of staining for electroporated neurons?

I am doing electropration in my neurons with some construct that has been tagged with GFP. Now I want to stain the neurons for Tau protein in red. I am getting very very faint or no staining at...

05 May 2016 7,334 11 View