I want to preserve bovine PBMCs for doing flow cytometry later on. It doesn't seem feasible to count PBMC cells for preservation as there will be lots of samples.

Is resuspending the PBMC pellets (after Sepmate tube extraction) in 1 ml of 10% DMSO 90% HS and freeze storage enough for keeping them viable long term?

Can i use FBS instead of HS?

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