Sulav Shrestha

5 Questions 5 Answers 0 Followers

Questions related from Sulav Shrestha

Will permeabilization of PBMCs in flow cytometry for extracellular markers make the results invalid?

I am conducting a multiplex flow cytometry assay to count the CD3, CD4, CD8 and CD21 markers in bovine PBMCs. However, the manufacturer of CD3 antibody recommends fixing the cells and...

10 February 2023 8,689 2 View

How to effectively extract DNA from dairy cattle's milk?

Does anyone have experience of extracting DNA out of dairy cow's milk? I am interested in the DNA present in the B-cells. I have done extraction from blood but want to try extraction from milk. I...

03 September 2020 3,738 5 View

In what concentration should i preserve bovine PBMC in 10% DMSO 90% HS for B-cell count via flow cytometry later on?

I want to preserve bovine PBMCs for doing flow cytometry later on. It doesn't seem feasible to count PBMC cells for preservation as there will be lots of samples. Is resuspending the PBMC pellets...

08 July 2020 8,467 2 View

Why am i seeing large variation in ds DNA concentration measurement in between Nanodrop and Qubit fluorometric quantitation?

I am experiencing wide variation in between the concentration reads from Nanodrop and Qubit Fluorometer for the same samples. The reads are mostly higher from Nanodrop which is sometimes in excess...

25 June 2020 4,921 2 View

What can be the highest milk fat level in dairy cows in post-milking samples?

What can be the maximum level of milk fat in dairy cows if the milk samples are taken post-milking by hand stripping? I am getting up to 20%! I understand that the milk fat level tends to increase...

17 September 2014 9,897 16 View