Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Hi i want to ask that in order to find out LC 50 (96 hours) of particular toxin for fish. Should we keep the animals with food or without food?

if we expose the fish after acclimatization to particular toxin in order to find out LC 50 (96 hours).Should we keep the fish off feed or with feed for that duration. Some literature show that...

24 February 2021 357 2 View

Why my Total RNA samples do not have clear two bands?

This is gel electrophoresis result for total RNA samples with good conc. However, I guess there is a problem with the purity of the samples, i can't figure out why it appears like this? And how...

22 February 2021 338 9 View

How COVID-19 has affected mental health?

The pandemic has led to depression and anxiety and its negative effects shouldn't be ignored. However, it has also led some people to become more resilient! What do you think? Thank you.

21 February 2021 4,733 19 View

Is nanotechnology part of materials science? Is it possible to obtain Pico technology and move from hypothesis to application?

Pico-scale This is three orders of magnitude smaller than a nanometer (and thus most nanotechnology) and two orders of magnitude smaller than most chemistry transformations and measurements. Pico...

18 February 2021 4,540 5 View

Is There are any idea to achieve H NMR signals for swollen compound ??

the samples are not completely soluble - just swelled the solvents that's mean the sample may be partially soluble any suggestion, please

16 February 2021 8,801 3 View

Pseudo random number generator in Matlab code ?

i want to implement pseudo random number generator in matlab ...i request to every one if someone have code these PRNG share with me SplitMix Permuted Congruential Generator (PCG) Random...

14 February 2021 6,828 7 View

Please ,How can I correct this kind of error?

27 January 2021 4,815 5 View

What is the best lighting design for conservation?

In the museums, the sensitive organic materials need to be displayed under 50 lux or less and aways from any UV or IR. LEDs still have a little amount of UV. What is the better option? Is it...

20 January 2021 5,965 3 View

No title

11 January 2021 3,284 3 View

How to extract DNA from faecal sample using DNeasy blood and tissue kit? We dont have DNA stool minikit?

Extraction of DNA from faecal sample

16 August 2020 9,250 1 View

Acceptable CV% and SD for results between stool samples in qPCR?

What would be the acceptable CV% and SD for copy number results between stool samples in qPCR (same group)? I am evaluating different bacteria (E.coli, Lactobacillus, etc.) using the absolute...

06 August 2020 8,112 4 View

Other ways to check DNA quality? How long can I store human stool samples?

Due to the pandemic, I can't go back to my university to conduct experiments. I have my stool samples and extraction kit with me in a smaller laboratory however they don't have the equipment I...

07 July 2020 5,172 1 View

Can we extract exosomes from stool using the Norgen Urine Exosome Purification kit?

After dilution of Fecal matter in PBS, the technician used the Noregen Urine Exosome Purification kit and RNA extraction. So my basic question is: Do you think we've got actual exosomes or just...

13 January 2020 1,420 2 View

Calculating sample size to assess horizontal gene transfer in-vivo (colon/stool samples) between two experimental groups and one control group?

The idea is to use rodents and to induce HGT within their microbiomes, one high exposure group, one low exposure and one control. The extent of HGT will then be assessed. What size would my sample...

31 December 2019 7,859 2 View

Can we extract exosomes from stool using the Norgen Urine Exosome Purification kit?

After dilution of Fecal matter in PBS, the technician used the Noregen Urine Exosome Purification kit and RNA extraction. So my basic question is: Do you think we've got actual exosomes or just...

31 December 2019 7,685 2 View

Long-term stability of inflammatory factors in human stool?

I've joined a new lab, and my PI just told me that they have human fecal samples on swabs that have been stored at 4 degrees Celsius for about two years. She asked me if I thought it would still...

10 November 2019 158 3 View

Same CT for diferent dilutions/concentrations?

I am working with DNA extracted from feces. I made a qPCR using concentrations of 100, 75, 50 and 20ng final. The CT value was very similar between dilutions between 29 and 30. My positive control...

10 November 2019 7,803 8 View

Faecal sample storage: which one is better, RNAlater or ethanol 95%?

I want to isolate DNA and RNA from feline faecal samples in order to determine which pathogens (viruses, bacteria, fungi, parasites...) are present in the samples. The isolation will not be...

22 September 2019 3,029 4 View

Are HEPA filters essential for DNA and RNA isolation?

I want to isolate DNA and RNA from faecal and blood samples in order to determine which pathogens (viruses, bacteria, fungi, parasites...) are present in the samples. I currently have one PCR...

08 September 2019 6,363 4 View