I would like to know why I obtained such false positive curves, which appears on both linear ang log scales healthy curves?

30 March 2024 0 6K Report

The positive curves for E coli different strains from mucual stool sample.

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How to fix background error in rietveld refinement of one XRD peak using GSAS-II?

I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II

05 August 2024 5,288 2 View

Why does the MFDFA algorithm need to calculate the profile of the time series?

As described in the Multifractal detrended fluctuation analysis (MFDFA) algorithm, it at first calculates the profile of the time series, and then other steps are operated on the profile....

05 August 2024 9,365 2 View

How to select target material for X-Ray generation?

Generally, the target material is Mo, Co, Cu, Fe, Cr, etc, so on what basis should we choose this material?

31 July 2024 5,824 0 View

Entropy measure and QSPR modeling in Graph Theor. How to construct the table for lengthy equation?

The entropy measured of molecular graphs plays a crucial rule. The network structures in some cases are very lengthy calculations to handle. Some author avoid to construct table where as most...

30 July 2024 3,125 0 View

Reason for discontinuities in my Band structure?

Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...

25 July 2024 2,918 11 View

Critique Calculaltion in Topological Indices and QSPR Modeling?

The first step is to analyze a 2D molecular graph and implement partitioning techniques to calculate the topological indices. Secondly impose statically tools to generate QSPR model, may or may...

24 July 2024 9,642 1 View

What strategy should be adopted to calculate topological indices efficiently?

Most of the time it is observed that in many manuscripts there are several typo error of topological calculation errors of calculation and partition of edges what ever the type of partitioning it...

22 July 2024 811 0 View

How I can add anew research in my account ?

Their is new research published in the biological control journal, and I am one of the authors. I want to add it to my account at researchgare. How can I do this?

21 July 2024 7,545 0 View

How to perform immunofluorescence on suspension cells?

Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...

20 July 2024 3,797 2 View

How to cacualte the creep life of polycrystal with CPFEM?

I saw a paper(PHAN V-T, ZHANG X, LI Y, et al. Microscale modeling of creep deformation and rupture in Nickel-based superalloy IN 617 at high temperature [J]. Mechanics of Materials, 2017, 114:...

16 July 2024 8,436 0 View

Qiagen QIAamp DNA Stool Kit or QIAamp PowerFecal DNA Kit?

Hello colleagues, I want to determine which kit is better for extracting DNA from insects in bird feces, with the purpose of conducting barcoding later on. So far, we have been using a modified...

07 February 2024 2,601 1 View

Mice Calprotectin ELISA kit (Stool)?

Have anyone tried to measure calprotectin from feces sample of mice using ELISA

03 February 2024 6,048 2 View

How do you collect Mouse fecal samples for microbiome analysis?

I am to collect mice fecal samples for microbiome analysis. I've been reading some articles where each mouse was placed in a cage and allowed to defecate for a few hours since it is ideal to use...

28 January 2024 5,465 1 View

How can I analysis single-gene level abundance in Shotgun sequencing data?

I'm doing analysis with shotgun sequencing data, not 16S, from human stool sample sequenced by Illumina Miseq. Usually I do BIobakery Humann pipeline, and get results of path coverage, path...

02 November 2023 8,084 1 View

I need a high-quality method for efficient extraction of acylcarnitines from fecal specimens?

Dear All, I need a high-quality method/protocol to efficiently extract acylcarnitines from fecal specimens for further LC-MS/MS analysis. This method must be feasible and removes the interfering...

28 October 2023 143 4 View

Why my stool DNA samples have A260/280 > 2?

Hello, I am extracting human DNA from stool samples using Quiagen´s QIAamp Fast DNA Stool Mini Kit. The DNA yield seems to be fine, but the A260/280 ratio is above 2.0 (2.1-2.2). What might be the...

28 September 2023 9,158 1 View

Protocol to prepare mouse fecal sample to measure cytokines by ELISA test?

Using a Milliplex kit I should measure 7 cytokines at once in mouse feces. I have doubts about how to prepare the stool sample because I have never done it before. Can you suggest a protocol if...

26 September 2023 9,542 0 View

Could someone please advise me of a reliable chemistry for assessing intestinal permeability in humans from blood or stool samples?

I plan to evaluate intestinal permeability from blood and stool samples, I am considering markers such as zonnulin or I-FABP through ELISA. if anyone has experience I would be grateful for any advice.

28 August 2023 7,526 0 View

What is the protocol for frozen Human stool for DNA extraction?

-20 C dilution

01 August 2023 197 0 View

Cryopreservative in long-term storage of stool samples for bacterial culturing?

Hi everyone, in line with this publication (Effect of cryopreservation medium conditions on growth and i... ) adding 20% glycerol or glycerol + media before storage at -80 C increases the number...

26 June 2023 4,335 1 View