I plan to evaluate intestinal permeability from blood and stool samples, I am considering markers such as zonnulin or I-FABP through ELISA. if anyone has experience I would be grateful for any advice.
As a stable element
23 July 2024 4,187 1 View
Hello, dear RG community. Personally, I have found Xarray to be excruciatingly slow, especially for big datasets and nonstandard operations (like a custom filtering function). The only suggestion...
15 July 2024 4,705 2 View
o impacto da Gestão Estratégica de Marketing, na tomada de decisões empresariais?
10 July 2024 3,429 0 View
Hi all, I am working on both primary hippocampal mouse neurons and i3Neurons to isolate axonal material. I plate them in microfluidic devices in house made (like any other you can find on the...
01 July 2024 8,070 0 View
Can you use the MTT assay of cell viability for tissue samples derived from rats e.g. liver, muscle, heart, brain, or is this method reserved for cultured cells? Thank you
29 May 2024 6,389 2 View
I was simulating 3 types of speed controller types for flying shears. P, PI and PD speed controller. And my question again is, what is the reason for not using PD control even if it can...
15 May 2024 4,806 3 View
Hello everyone, I am currently exploring several options to give the collected data the greatest value possible. I have demographic data on older people, where I perform various memory and mood...
02 May 2024 876 6 View
Could you please provide me some authoritetic refers where this formula used if it accurate OR tell me why it is wrong and how to fix it? 1. TOA (L) = ML * Qcal + AL 2. BT = (K2 / (ln (K1 / L) +...
19 April 2024 8,924 0 View
Hello, dear RG community. Very often, gas fraction (also known as void fraction or gas holdup) is thought of as a ratio of the gas volume to the total volume:...
04 April 2024 7,290 0 View
Dear Researchers, I am reaching out to this esteemed community seeking insights into a recent experiment I conducted involving cell seeding in agarose micro-wells. My aim is to engage with fellow...
22 March 2024 4,438 0 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,418 1 View
Difficulty with permittivitt and Magnetic Permeability Calculations Hello everyone, I have all the parameters related to the calculations of the permittivitty and magnetic permeability...
30 July 2024 5,206 1 View
I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?
29 July 2024 3,138 2 View
Dear Siesta Users, I installed Siesta 5.0.1 and I want to use Grimme's D3 correction. According to the 5.0.1 manual, I could use a few parameters to include the D3 correction in the...
27 July 2024 5,747 0 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View
I run mechanochemical reactions that involves the use of a large amount of catalyst (1:1 molar ratio to the reactant). When I try to understand the effect of amount of catalyst on the reaction, I...
23 July 2024 9,938 1 View
Hi, I am a PhD student working at Jagiellonian University, Faculty of Chemistry. My work is related to the development of new analytical methods for the quantification of short-chain fatty acids...
22 July 2024 5,492 6 View
Dear Researchers, My question is associated with the "MDCI" method in Orca. Please let me clarify my question using a simple example: Suppose we are going to perform CBS extrapolation using "!...
21 July 2024 1,632 0 View
This is a slice of intestinal tissue from the Chinese mitten crab (intestine in the upper right corner). I injected WSSV into this horseshoe crab. Now I want to know what these red spots next to...
18 July 2024 5,602 0 View