Mice Calprotectin ELISA kit (Stool)?

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Cyclization should be performed before mutation or after mutation of a linear peptide sequence by in-silico method?

I have a doubt on in-silico modeling. I want to cyclize a linear peptide sequence by in silico. Which is more favorable method I don't understand. There is 2 pathways- 1. I can cyclize a linear...

12 June 2024 2,449 2 View

What cDNA dilution can be used for qPCR reaction?

I have obtained 100 ul of pre-amplified cDNA using the SSIV SINGLE CELL PRE-AMP KIT. Now i want to make a dilution because my samples are limited and i cannot directly use the cDNA. So what...

22 May 2024 6,017 3 View

How can I calculate average dG MM/GBSA energy from selected frames of MD trajectories?

I need to calculate dG MM/GBSA energy through the MD simulation trajectories. From RMSD values I selected last 400 frames from MD trajectories. I ran thermal_mmgbsa.py script, but it calculates dG...

22 May 2024 10,076 5 View

How is object segmentation and object detection are different?

How YOLOv8 is used to obtain the contours/object and calculate individual area

02 May 2024 5,395 3 View

Is it necessary to change the medium every 24 hours after drug treatment in cell line study and provide fresh drug to the cell?

I am keeping my cell line for drug treatment and monitoring its effect at 24 hrs, 48 hrs and 72 hrs? Should I change the medium every 24 hrs and give fresh drug treatment or just leave the medium...

01 May 2024 10,011 3 View

Can anyone explain the N/P charge ratio of protein DNA complex during LLPS formation?

Protein:DNA charge ratio plays an important role in forming condensates. In most cases the N/P ratio ~1 is favored. However how this ratio depends on the concentration of the protein is not...

20 April 2024 4,752 0 View

How BLUE ECONOMY can upgrade the economic profile of the nations?

The "blue economy" refers to the sustainable use of ocean resources for economic growth, improved livelihoods, and jobs while preserving the health of ocean ecosystems. It encompasses a wide range...

19 April 2024 7,455 2 View

What could be the possible reason for this different microstructure?

I've prepared the following ceramics: Sample ID = Composition LMT = Li2Mg3TiO6 LMCT = Li2(Mg2.88Ca0.12)TiO6 LMCT-MN = Li2(Mg2.88Ca0.12)(Ti0.95(Mg1/3Nb2/3)0.05)O6 LMT-MN =...

07 April 2024 4,716 2 View

How AI can assist in the progress of Oceanography?

AI has immense potential to address various aspects of climate change by enhancing our understanding of the climate system, optimizing resource usage, and developing innovative solutions. Here are...

26 March 2024 958 1 View

After formulating your hypothesis based on a problem statement , what are the important steps constructed within DS project life cycle ?

Please explain the importance of : 1. DOE - Design Of Experiment 2. Statistical Testing Process Document

12 March 2024 1,554 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,081 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,156 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,706 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 761 1 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,121 4 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,482 5 View