Mice Calprotectin ELISA kit (Stool)?

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How to extract protein after trizol RNA and back extraction of genomic DNA?

I have limited amount of samples, so I am trying to get RNA, DNA and protein from the same samples. I used Trizol reagent for RNA isolation, then use back extraction buffer for genomic DNA...

13 January 2021 1,566 2 View

Where can I find solar irradiation data with high resolution?

I am trying to generate a 1 day solar irradiation profile. Any suggested high resolution(possibly in minutes) source for the same?

06 December 2020 5,976 18 View

How to implement only a Gate with activation sigmoid or tanh in keras?

I want to implement only a Gate similar to forget gate or output gate of LSTM cell. Mind it...!!! I am not implementing the entire LSTM cell rather I have certain input and previous output and I...

22 September 2020 8,236 3 View

Could someone help me interpret the following PMF curve, especially the physical reason behind the multiple potential wells?

I am simulating the pulling of two nanoparticles in a polymer. I get the attached PMF curve, showing a secondary (and much deeper) potential well. I was wondering if there is a physical...

19 September 2020 7,504 5 View

How to determine relationship among the independent variables to get lowest MSE (Mean Squared Error) in prediction?

I have a dependent variable A, which can be perceived from B, C and D. So to say A = function(B,C,D). B, C, D results in A that is known to us. However, I don't know whether B,C and D follow a...

28 June 2020 6,332 6 View

IS it Necessary for Primers in Taq-Man PCR to be HPLC Purified?

I want to set up a taq man reaction. I want to know that in taq man reaction I have to order HPLC purified Primers or ordinary primers are adequate for me. If you have experience in taq man,...

23 June 2020 5,511 3 View

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We're looking at storing serum and plasma samples from a clinical trial. I've looked at various papers, but the only place where I found some hazy answer was in the supplementary. But I'm hoping,...

04 March 2020 8,858 3 View

How to calculate the required motor HP to run a machine?

Hello every one, Can some body help me in calculating the required HP for motor to run the machine which includes transmission system with 2 Nos of shaft and cutting blades.

28 December 2019 9,788 3 View

Is there any benchmark dataset for distributed camera networks?

I am particularly looking for dataset containing cameras capturing videos in a distributed setup. The videos, captured by multiple cameras have to be correlated. Is there any benchmark dataset on...

22 November 2019 9,567 3 View

Could anyone recommend a protocol to estimate the concentration of residual antibiotics in a tissue allograft?

I have been trying to detect and quantify residual antibiotics left over from processing of my tissue allografts. Could anyone help me with the same?

01 August 2019 8,465 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View