Hi everyone,
in line with this publication (Article Effect of cryopreservation medium conditions on growth and i...
)adding 20% glycerol or glycerol + media before storage at -80 C increases the number of culturable bacteria from feces. Does anyone have experience with long-term storage with cryopreservatives?
Any information is helpful :)
When you are ready to culture the stored samples, thaw them by transferring the cryovials from the -80°C freezer to a 37°C water bath or under room temperature conditions. Use appropriate sterility precautions during thawing and subsequent culturing processes.
It is important to note that different bacterial species may have different sensitivity to cryopreservation and may require specific modifications or optimizations in the cryopreservation protocol.
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