Protocol to prepare mouse fecal sample to measure cytokines by ELISA test?

27 September 2023 0 10K Report

Using a Milliplex kit I should measure 7 cytokines at once in mouse feces. I have doubts about how to prepare the stool sample because I have never done it before. Can you suggest a protocol if you have already done a similar test?

Badges
Science topic

Similar topics
Clinical Pharmacology
Dosing

More Michele Biagioli's questions See All

How to name this rock?

Dear RG community members, I have found this sample in our rock collection used with bachelor students enrolled in Geology studies. I do not know where this rock comes from. I have made a thin...

17 June 2024 9,525 23 View

How did this rock form?

Dear RG members, I have produced a thin section of a rock in our rock collection, used for students enrolled in the second year of the bachelor of Geology at Sapienza University of Rome....

17 June 2024 689 3 View

What is the best buffer to resuspend proteins extracted from oil?

I hope everyone is well! I extracted proteins from sunflower and olive oils using acetone and then washing with n-hexane. I used RIPA buffer to resuspend because I plan to dot blot these samples....

02 June 2024 10,007 1 View

Crestomer on Plywood?

Normally unsaturated polyester or more in general radicalic bonding pastes doesn't adhere to marine plywood, due to the inhibition effect of formaldehyde and tannins. Crestomer 1152 of Scott...

22 April 2024 1,988 0 View

How can rotational stiffness be easily calculated for a steel connection?

I am currently working on modeling a steel structure with bolted connections, and I'm looking to incorporate the suitable rotational stiffness k. However, I've found that reference codes like EN...

25 January 2024 4,895 0 View

Friction at negative loads in viscoelastic materials?

dear friends I was playing with some theory of friction in viscoelastic solids, perhaps you can help me finding if there is any research on friction at negative loads (therefore under adhesive...

28 November 2023 5,133 1 View

Does exist researches on shoulder ultrasound for GP's?

24 November 2023 8,374 1 View

Why olivine is so commonly transformed in calcite?

Basaltic (or generically MgO-rich igneous rocks) have olivine phenocrysts commonly replaced by calcite, expecially if found in submarine environments. Of course this is not the only way to...

18 October 2023 1,428 1 View

Why is my AKTA Pure showing protein concentrations lower than expected?

I've recently purified TEV with a his-tag on a Ni-NTA column using our AKTA Pure FPLC. It usually works great. This time I had two peaks of lower absorbance over many fractions in the elution...

27 August 2023 7,415 0 View

Abstract submitted with wrong name?

Hi, I'm writing to make you know that many abstracts from AOU San Luigi Gonzaga University of Medicine (Turin) were submitted by the co-author "Marco Ortenzi". This was a mistake because I'm...

13 August 2023 3,677 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

What is the best sampling strategy?

I am conducting a qualitative study that uses interviews to investigate the perceptions of teachers about a particular leadership practice and I am focusing on 3 schools which have a total number...

01 August 2024 8,457 10 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View