I need a detailed report to this cell line B lymphoblastoid cell line SKW 6.4?

Hi All, I need to know whether this cell line (SKW 6.4), is a transformed B lymphoid cell line or not, since some reports showed that they are transformed with EPV! Thank you so much Montaser

02 March 2018 5,972 3 View

Un-explained elevation in VEGF after treatment with Fenofibrate ?

03 April 2017 7,242 2 View

What is the advantage to have a 6-thioguanine resistant cell line ?

Excuse me I need to know the exact advantage for having a cell line that is resistant to the 6-Thioguanine?

02 March 2017 9,189 3 View

What is the most suitable histology stain to visualize fibrin in tissue sections?

01 February 2017 6,209 8 View

This vehicle (10% DMSO, 15% Solutol, 75% water for injection) didn't 100mg/kg Fenofibrate ?

as you know fenofibrate is a poorly water soluble drug .... i tried to use one of the suggested published vehicle which is 10%DMSO, 15%Solutol, 75% Sterile water for injection ... but...

10 November 2016 6,352 3 View

Could i give mice ethyl acetate Intraperitoneal injection ?

08 September 2016 6,761 3 View

How we can give the required dose of Fenofibrate without exceeding the Toxic dose of it's solvent ?

I am working on the effect of fenofibrate on the 4T1 breast cancer metastasis by using Balb/c mice as the animal model ... but I have a problem in how I could give the required dose of fenofibrate...

07 August 2016 5,433 1 View

I would like to ask if i can use Evans blue stain with cancer cells inoculated through the tail vein ?

Hi ... could i mix Evans blue stain with 4T1 cells when i want to inoculate them through the mice tail vein to ensure the right IV inoculation ?

01 January 1970 10,041 1 View

I have a problem in exceeding the lethal dose of DMSO ...because it is the solvent of my required drug Fenofibrate ...any suggestion please ???

I am working on the effect of fenofibrate on the tumor cell metastasis by using Balb/c mice as the animal model. Now I have a problem which it's the fenofibrate is not soluble in water, so I used...

01 January 1970 3,079 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View