Hi ... could i mix Evans blue stain with 4T1 cells when i want to inoculate them through the mice tail vein to ensure the right IV inoculation ?
@mohamed mahday ... Thank u very much ^_^
Hi All, I am going to infect suspension cell line (SKW, Normal B-Lymphocytes) with mutant K-RAS, I would like to ask for advises to increase the infection efficiency of the suspension...
02 March 2018 2,082 2 View
Hi All, I need to know whether this cell line (SKW 6.4), is a transformed B lymphoid cell line or not, since some reports showed that they are transformed with EPV! Thank you so much Montaser
02 March 2018 6,082 3 View
Fenofibrate treatment is found to decrease the level of VEGF in hyperlipidemic patients ...and this was the rationale for testing its effect on angiogenesis in cancer patients. Surprisingly VEGF...
03 April 2017 7,358 2 View
Excuse me I need to know the exact advantage for having a cell line that is resistant to the 6-Thioguanine?
02 March 2017 9,310 3 View
i would like to see the fibrin that is associated with metastatic cancer cells and i would like to know which is the best stain that could be used to stain fibrin
01 February 2017 6,328 8 View
as you know fenofibrate is a poorly water soluble drug .... i tried to use one of the suggested published vehicle which is 10%DMSO, 15%Solutol, 75% Sterile water for injection ... but...
10 November 2016 6,496 3 View
I am going to use ethyl acetate as a solvent to the Fenofibrate ... according to what I read, ethyl acetate could be given orally or by inhalation but I would like to ask if it could be...
08 September 2016 6,912 3 View
I am working on the effect of fenofibrate on the 4T1 breast cancer metastasis by using Balb/c mice as the animal model ... but I have a problem in how I could give the required dose of fenofibrate...
07 August 2016 5,598 1 View
I am working on the effect of fenofibrate on the tumor cell metastasis by using Balb/c mice as the animal model. Now I have a problem which it's the fenofibrate is not soluble in water, so I used...
01 January 1970 3,187 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...
03 August 2024 5,828 4 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View