I need a detailed report to this cell line B lymphoblastoid cell line SKW 6.4?

Hi All, I need to know whether this cell line (SKW 6.4), is a transformed B lymphoid cell line or not, since some reports showed that they are transformed with EPV! Thank you so much Montaser

02 March 2018 5,972 3 View

I would like to ask for advises to increase the infection efficiency of suspension cells with mutant K-RAS?

Hi All, I am going to infect suspension cell line (SKW, Normal B-Lymphocytes) with mutant K-RAS, I would like to ask for advises to increase the infection efficiency of the suspension...

02 March 2018 1,970 2 View

Un-explained elevation in VEGF after treatment with Fenofibrate ?

03 April 2017 7,242 2 View

What is the advantage to have a 6-thioguanine resistant cell line ?

Excuse me I need to know the exact advantage for having a cell line that is resistant to the 6-Thioguanine?

02 March 2017 9,189 3 View

What is the most suitable histology stain to visualize fibrin in tissue sections?

01 February 2017 6,209 8 View

This vehicle (10% DMSO, 15% Solutol, 75% water for injection) didn't 100mg/kg Fenofibrate ?

as you know fenofibrate is a poorly water soluble drug .... i tried to use one of the suggested published vehicle which is 10%DMSO, 15%Solutol, 75% Sterile water for injection ... but...

10 November 2016 6,352 3 View

Could i give mice ethyl acetate Intraperitoneal injection ?

08 September 2016 6,761 3 View

How we can give the required dose of Fenofibrate without exceeding the Toxic dose of it's solvent ?

I am working on the effect of fenofibrate on the 4T1 breast cancer metastasis by using Balb/c mice as the animal model ... but I have a problem in how I could give the required dose of fenofibrate...

07 August 2016 5,433 1 View

I have a problem in exceeding the lethal dose of DMSO ...because it is the solvent of my required drug Fenofibrate ...any suggestion please ???

I am working on the effect of fenofibrate on the tumor cell metastasis by using Balb/c mice as the animal model. Now I have a problem which it's the fenofibrate is not soluble in water, so I used...

01 January 1970 3,079 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View