I am working on the effect of fenofibrate on the tumor cell metastasis by using Balb/c mice as the animal model. Now I have a problem which it's the fenofibrate is not soluble in water, so I used stock DMSO as a solvent. The required dose of FF was100mg/1000g, so I will need to prepare this dose by using a high amount of DMSO because the solubility of FF in DMSO IS 15mg/ml. AlL mice were severely tired after being injected with this dose! Btw the control group also were died after being injected with DMSO alone !!!! any suggestions please!
Did you try methanol as a solvant: you can go MAX at 10% vol / vol injection with this solvant.
You can also reduce DMSO at an acceptable standard concentration for in vivo experimen, using ultrasonication, and injecting your "solution" (which is not) intra peritoneally. The i.p. route, which cannot be used in clinics (excepted in some cases, e.g. ascitic ovarian cancer), usually greatly help phamacologists...
Robert
Hello Montaser,
Your problem is very complex, from above description even for an animal of 75g you need 7.5mg FF which is soluble in 500ul DMSO and you can't inject 500ul DMSO to an animal. The best solution to your problem, which I can see from my personal experience is to use a double solvent system. First of all, try to dissolve your drug in ethanol. If it dissolves then it's good. If it doesn't you can use ethanol and DMSO in mix. eg. For 1ml, you can use 800ul eth+ 200ul DMSO or even 900ul eth+ 100ul DMSO. If that dissolves your 15mg drug then amount of DMSO delivered to animal will be greatly reduced. As per my experience, this system should dissolve the drug well . Please give it a try and do let me know if it works..
Hope it helps !
YOU MAY TRY TO OBTAIN HIGH CONCENTRATION OF THE DRUG DİSSOLVE AS STOCK and then the other dilution you may use ethanol.
best,
- Badges
- Science topic
- Similar topics
- Medicine
- Disease
- Disease Models
- Animal Models