I need a detailed report to this cell line B lymphoblastoid cell line SKW 6.4?

Hi All, I need to know whether this cell line (SKW 6.4), is a transformed B lymphoid cell line or not, since some reports showed that they are transformed with EPV! Thank you so much Montaser

02 March 2018 5,972 3 View

I would like to ask for advises to increase the infection efficiency of suspension cells with mutant K-RAS?

Hi All, I am going to infect suspension cell line (SKW, Normal B-Lymphocytes) with mutant K-RAS, I would like to ask for advises to increase the infection efficiency of the suspension...

02 March 2018 1,970 2 View

What is the advantage to have a 6-thioguanine resistant cell line ?

Excuse me I need to know the exact advantage for having a cell line that is resistant to the 6-Thioguanine?

02 March 2017 9,189 3 View

What is the most suitable histology stain to visualize fibrin in tissue sections?

01 February 2017 6,209 8 View

This vehicle (10% DMSO, 15% Solutol, 75% water for injection) didn't 100mg/kg Fenofibrate ?

as you know fenofibrate is a poorly water soluble drug .... i tried to use one of the suggested published vehicle which is 10%DMSO, 15%Solutol, 75% Sterile water for injection ... but...

10 November 2016 6,352 3 View

Could i give mice ethyl acetate Intraperitoneal injection ?

08 September 2016 6,761 3 View

How we can give the required dose of Fenofibrate without exceeding the Toxic dose of it's solvent ?

I am working on the effect of fenofibrate on the 4T1 breast cancer metastasis by using Balb/c mice as the animal model ... but I have a problem in how I could give the required dose of fenofibrate...

07 August 2016 5,433 1 View

I would like to ask if i can use Evans blue stain with cancer cells inoculated through the tail vein ?

Hi ... could i mix Evans blue stain with 4T1 cells when i want to inoculate them through the mice tail vein to ensure the right IV inoculation ?

01 January 1970 10,041 1 View

I have a problem in exceeding the lethal dose of DMSO ...because it is the solvent of my required drug Fenofibrate ...any suggestion please ???

I am working on the effect of fenofibrate on the tumor cell metastasis by using Balb/c mice as the animal model. Now I have a problem which it's the fenofibrate is not soluble in water, so I used...

01 January 1970 3,079 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View