I would like to ask for advises to increase the infection efficiency of suspension cells with mutant K-RAS?

Hi All, I am going to infect suspension cell line (SKW, Normal B-Lymphocytes) with mutant K-RAS, I would like to ask for advises to increase the infection efficiency of the suspension...

02 March 2018 1,970 2 View

Un-explained elevation in VEGF after treatment with Fenofibrate ?

03 April 2017 7,242 2 View

What is the advantage to have a 6-thioguanine resistant cell line ?

Excuse me I need to know the exact advantage for having a cell line that is resistant to the 6-Thioguanine?

02 March 2017 9,189 3 View

What is the most suitable histology stain to visualize fibrin in tissue sections?

01 February 2017 6,209 8 View

This vehicle (10% DMSO, 15% Solutol, 75% water for injection) didn't 100mg/kg Fenofibrate ?

as you know fenofibrate is a poorly water soluble drug .... i tried to use one of the suggested published vehicle which is 10%DMSO, 15%Solutol, 75% Sterile water for injection ... but...

10 November 2016 6,352 3 View

Could i give mice ethyl acetate Intraperitoneal injection ?

08 September 2016 6,761 3 View

How we can give the required dose of Fenofibrate without exceeding the Toxic dose of it's solvent ?

I am working on the effect of fenofibrate on the 4T1 breast cancer metastasis by using Balb/c mice as the animal model ... but I have a problem in how I could give the required dose of fenofibrate...

07 August 2016 5,433 1 View

I would like to ask if i can use Evans blue stain with cancer cells inoculated through the tail vein ?

Hi ... could i mix Evans blue stain with 4T1 cells when i want to inoculate them through the mice tail vein to ensure the right IV inoculation ?

01 January 1970 10,041 1 View

I have a problem in exceeding the lethal dose of DMSO ...because it is the solvent of my required drug Fenofibrate ...any suggestion please ???

I am working on the effect of fenofibrate on the tumor cell metastasis by using Balb/c mice as the animal model. Now I have a problem which it's the fenofibrate is not soluble in water, so I used...

01 January 1970 3,079 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View