I need a detailed report to this cell line B lymphoblastoid cell line SKW 6.4?

Hi All, I need to know whether this cell line (SKW 6.4), is a transformed B lymphoid cell line or not, since some reports showed that they are transformed with EPV! Thank you so much Montaser

02 March 2018 5,972 3 View

I would like to ask for advises to increase the infection efficiency of suspension cells with mutant K-RAS?

Hi All, I am going to infect suspension cell line (SKW, Normal B-Lymphocytes) with mutant K-RAS, I would like to ask for advises to increase the infection efficiency of the suspension...

02 March 2018 1,970 2 View

Un-explained elevation in VEGF after treatment with Fenofibrate ?

03 April 2017 7,242 2 View

What is the advantage to have a 6-thioguanine resistant cell line ?

Excuse me I need to know the exact advantage for having a cell line that is resistant to the 6-Thioguanine?

02 March 2017 9,189 3 View

This vehicle (10% DMSO, 15% Solutol, 75% water for injection) didn't 100mg/kg Fenofibrate ?

as you know fenofibrate is a poorly water soluble drug .... i tried to use one of the suggested published vehicle which is 10%DMSO, 15%Solutol, 75% Sterile water for injection ... but...

10 November 2016 6,352 3 View

Could i give mice ethyl acetate Intraperitoneal injection ?

08 September 2016 6,761 3 View

How we can give the required dose of Fenofibrate without exceeding the Toxic dose of it's solvent ?

I am working on the effect of fenofibrate on the 4T1 breast cancer metastasis by using Balb/c mice as the animal model ... but I have a problem in how I could give the required dose of fenofibrate...

07 August 2016 5,433 1 View

I would like to ask if i can use Evans blue stain with cancer cells inoculated through the tail vein ?

Hi ... could i mix Evans blue stain with 4T1 cells when i want to inoculate them through the mice tail vein to ensure the right IV inoculation ?

01 January 1970 10,041 1 View

I have a problem in exceeding the lethal dose of DMSO ...because it is the solvent of my required drug Fenofibrate ...any suggestion please ???

I am working on the effect of fenofibrate on the tumor cell metastasis by using Balb/c mice as the animal model. Now I have a problem which it's the fenofibrate is not soluble in water, so I used...

01 January 1970 3,079 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View