I'm working with some hypothetical proteins, can I classify them or just predict their functions base on homology?

Classification of hypothetical proteins.

06 July 2016 7,044 2 View

By my calculations, I can use a 2X PCR master mix 100 reactions (50uL) for 500 reactions (10uL), am I right?

05 June 2016 9,328 6 View

I got an error message 'ID#68 Error' while trying to run blast with multiple sequences. What does it mean and what do I do to get results?

Message ID#68 Error: Error occurred while trying to set up a Blast Object from CGI context: CFastaReader: Near line 1, there's a line that doesn't look like plausible data, but it's not marked as...

05 June 2016 7,767 6 View

I want to sequence a gene (SORBIDRAFT_01g047430) which happens to be a putative gene, what can you say about sequencing a putative gene?

I want to sequence a putative gene

03 April 2016 8,718 3 View

Can phytic acid content be calculated from total phosphorous content?

02 March 2016 2,859 2 View

How do I order for AFLP and SSR primers and other molecular biology reagents?

I am want to know how to order for AFLP and SSR primers as well as other molecular biology reagents

10 November 2015 1,999 0 View

Is it trendy to make a checklist of bioinformatics tools? Will it be publishable?

Bioinformatics tools and applications

02 March 2015 7,146 3 View

Anyone interested in a partnership to examine the genetics of plant height?

02 March 2015 2,938 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View