I'm working with some hypothetical proteins, can I classify them or just predict their functions base on homology?

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I got an error message 'ID#68 Error' while trying to run blast with multiple sequences. What does it mean and what do I do to get results?

Message ID#68 Error: Error occurred while trying to set up a Blast Object from CGI context: CFastaReader: Near line 1, there's a line that doesn't look like plausible data, but it's not marked as...

05 June 2016 8,948 6 View

By my calculations, I can use a 2X PCR master mix 100 reactions (50uL) for 500 reactions (10uL), am I right?

I want to reduce the cost of running my PCR reactions by scaling down to 10uL final volume. I want do this by using OneTaq Quick-Load 2X MasterMix with Standard Buffer - 100 reactions (50uL).

05 June 2016 9,483 6 View

I want to use CRISPR/Cas9 on three regions of SORBIDRAFT_01g047430, do I amplify and sequence the whole gene or just my target regions?

Use of CRISPR/Cas9 on SORBIDRAFT_01g047430 and design of gRNAs.

04 May 2016 8,474 3 View

I want to sequence a gene (SORBIDRAFT_01g047430) which happens to be a putative gene, what can you say about sequencing a putative gene?

I want to sequence a putative gene

03 April 2016 8,852 3 View

Can phytic acid content be calculated from total phosphorous content?

I want to assay for phytic acid content in Sorghum bicolor seeds but the lab I contacted for the work says they can only assay for total phosphorous content.

02 March 2016 3,021 2 View

How do I order for AFLP and SSR primers and other molecular biology reagents?

I am want to know how to order for AFLP and SSR primers as well as other molecular biology reagents

10 November 2015 2,129 0 View

Is it trendy to make a checklist of bioinformatics tools? Will it be publishable?

Bioinformatics tools and applications

02 March 2015 7,274 3 View

Anyone interested in a partnership to examine the genetics of plant height?

I'm examining the genetics of plant height using model plants as guinea pigs, and the possible application of findings on ornamental trees and shrubs, and food crops and trees. I have being...

02 March 2015 3,068 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Problems with ion exchange chromatography?

I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...

27 July 2024 6,605 0 View

How do regenerate Amberlite XAD 4 resin for further cycles of use for removing 0.5% Sarkosyl from recombinant protein solutions?

I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...

25 July 2024 5,001 2 View

How do nutrient transporters and aquaporins interact under abiotic stress in plants?

"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...

23 July 2024 9,109 0 View

How can we conduct DFT for solutions or mixtures ?

Can we conduct DFT studies for Ionic Liquids with Amines at various concentrations? If so, how can we proceed with these studies? Additionally, what parameters, besides IR, NMR, and HOMO-LUMO, can...

18 July 2024 319 1 View

Consequences of excess bases in the MCS after digestion?

Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...

17 July 2024 2,787 4 View

I have NMR,Mass spec,IR and uv of a mixture of three unknown compounds from natural products.I am attaching the data who can help me elucidate?

I isolated a mixture of three compounds from the stem bark of a plant . I have NMR, Mass spec, IR, and UV data. There are a lot of things I can't make meaning out of. Kindly help me with the...

02 July 2024 9,531 4 View

List of journals impact factors?

Dear colleagues, Is it possible to send me the list of journals impact factor for the year 2024 (classification is for the year 2023)? excel format if it is possible. Thank you in...

29 June 2024 2,102 3 View