By my calculations, I can use a 2X PCR master mix 100 reactions (50uL) for 500 reactions (10uL), am I right?

05 June 2016 9,328 6 View

I got an error message 'ID#68 Error' while trying to run blast with multiple sequences. What does it mean and what do I do to get results?

Message ID#68 Error: Error occurred while trying to set up a Blast Object from CGI context: CFastaReader: Near line 1, there's a line that doesn't look like plausible data, but it's not marked as...

05 June 2016 7,767 6 View

I want to use CRISPR/Cas9 on three regions of SORBIDRAFT_01g047430, do I amplify and sequence the whole gene or just my target regions?

Use of CRISPR/Cas9 on SORBIDRAFT_01g047430 and design of gRNAs.

04 May 2016 8,350 3 View

I want to sequence a gene (SORBIDRAFT_01g047430) which happens to be a putative gene, what can you say about sequencing a putative gene?

I want to sequence a putative gene

03 April 2016 8,718 3 View

Can phytic acid content be calculated from total phosphorous content?

02 March 2016 2,859 2 View

How do I order for AFLP and SSR primers and other molecular biology reagents?

I am want to know how to order for AFLP and SSR primers as well as other molecular biology reagents

10 November 2015 1,999 0 View

Is it trendy to make a checklist of bioinformatics tools? Will it be publishable?

Bioinformatics tools and applications

02 March 2015 7,146 3 View

Anyone interested in a partnership to examine the genetics of plant height?

02 March 2015 2,938 2 View

Why should we choose deep learning to machine learning? Why is CNN better for image classification? Can I get Typical Answer?

What Characteristics makes CNN work better?

03 March 2021 1,458 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Is there a classification scheme for rock cavities or caverns with respect to their size and shape?

The range of forms and sizes of cavities in rock is very big. Is there a classification scheme for rock cavities regarding their size and shape?

02 March 2021 4,511 3 View

Image Processing and classification using keras/Tensorflow?

Do you have some recommendations approaches on Image processing and classification of plant diseases using TensorFlow/Keras?

02 March 2021 1,493 4 View

How to find the mode of classified ocean colour images?

Hello, I have classified 10 S3 OLCI images from the same area (althouth not the same size and/or quadrant) and I would like to do a final image using the mode of the pixel of these 10 images. I...

01 March 2021 9,874 3 View

Does anyone have kinetic and kinematic dataset for gait classification?

I want to differentiate between normal and abnormal movement. For this reason, I am looking forward to this type of dataset.

01 March 2021 9,485 1 View

How to classify strictly upper triangular matrices by similarity?

Let F be a field. Consider U, the set of n times n strictly upper triangular matrices in F. For X, Y in U, we call them similar if there exists some S, which is non-degenerate and upper...

01 March 2021 2,957 8 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View