I am want to know how to order for AFLP and SSR primers as well as other molecular biology reagents
Classification of hypothetical proteins.
06 July 2016 7,170 2 View
Message ID#68 Error: Error occurred while trying to set up a Blast Object from CGI context: CFastaReader: Near line 1, there's a line that doesn't look like plausible data, but it's not marked as...
05 June 2016 8,948 6 View
I want to reduce the cost of running my PCR reactions by scaling down to 10uL final volume. I want do this by using OneTaq Quick-Load 2X MasterMix with Standard Buffer - 100 reactions (50uL).
05 June 2016 9,483 6 View
Use of CRISPR/Cas9 on SORBIDRAFT_01g047430 and design of gRNAs.
04 May 2016 8,474 3 View
I want to sequence a putative gene
03 April 2016 8,852 3 View
I want to assay for phytic acid content in Sorghum bicolor seeds but the lab I contacted for the work says they can only assay for total phosphorous content.
02 March 2016 3,021 2 View
Bioinformatics tools and applications
02 March 2015 7,274 3 View
I'm examining the genetics of plant height using model plants as guinea pigs, and the possible application of findings on ornamental trees and shrubs, and food crops and trees. I have being...
02 March 2015 3,068 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope...
02 August 2024 7,843 0 View