I'm working with some hypothetical proteins, can I classify them or just predict their functions base on homology?

Classification of hypothetical proteins.

06 July 2016 7,044 2 View

By my calculations, I can use a 2X PCR master mix 100 reactions (50uL) for 500 reactions (10uL), am I right?

05 June 2016 9,328 6 View

I got an error message 'ID#68 Error' while trying to run blast with multiple sequences. What does it mean and what do I do to get results?

Message ID#68 Error: Error occurred while trying to set up a Blast Object from CGI context: CFastaReader: Near line 1, there's a line that doesn't look like plausible data, but it's not marked as...

05 June 2016 7,767 6 View

I want to use CRISPR/Cas9 on three regions of SORBIDRAFT_01g047430, do I amplify and sequence the whole gene or just my target regions?

Use of CRISPR/Cas9 on SORBIDRAFT_01g047430 and design of gRNAs.

04 May 2016 8,350 3 View

I want to sequence a gene (SORBIDRAFT_01g047430) which happens to be a putative gene, what can you say about sequencing a putative gene?

I want to sequence a putative gene

03 April 2016 8,718 3 View

How do I order for AFLP and SSR primers and other molecular biology reagents?

I am want to know how to order for AFLP and SSR primers as well as other molecular biology reagents

10 November 2015 1,999 0 View

Is it trendy to make a checklist of bioinformatics tools? Will it be publishable?

Bioinformatics tools and applications

02 March 2015 7,146 3 View

Anyone interested in a partnership to examine the genetics of plant height?

02 March 2015 2,938 2 View

No title

02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View