Instead of using falcon tubes, you can use microcentrifuge tubes that fit into a standard microcentrifuge. These tubes typically have smaller capacities (e.g., 1.5 mL or 2 mL), but they are suitable for processing smaller volumes of sample.

Here are a few examples of commercially available SOD assay kits:

Sigma-Aldrich SOD Assay Kit:

Cayman Chemical Superoxide Dismutase (SOD) Assay Kit:

BioVision SOD Activity Assay Kit:

Abcam Superoxide Dismutase (SOD) Activity Assay Kit:

These kits typically come with detailed protocols and all the necessary reagents for performing the assay.

Ankit Negi I have protocol for 3 mL mixture for 50% NBT reduction. So Can I reduce the volume to 2mL or 1.5mL and then I have to prepare the mixture components accordingly? Like 20mM x-chemical in mixture for 3mL and if I need to reduce the volume then I have to make the concentration to half (10mM)?

Bhawna Bhawna Follow the protocol for 3 mL. At the time of centrifugation, simply separate the mixture into two 1.5 mL portions in Eppendorf tubes.

There are SOD assay kits commercially available (we produce one). Sigma, Biovision and Abcam kits mentioned above are all the same, very similar to ours. They require much smaller samples than your current setup.