Why SOD of samples are higher than control?

10 May 2024 1 9K Report

Hello

I'm measuring SOD activity in plant leaves using NBT method. Reaction solution contains EDTA, riboflavin, methionine and PBS.

But my samples values are higher than control. Do you know how I can solve it. Thank you.

Kajari Das

To isolate SOD from other interfering compounds the centrifuged homogenate should be passed through a Sephadex column. The aliquots collected to be tested for SOD activity.

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