Gene microarray database for cancer patients

More Patrick Aouad's questions See All

Can cancer cells eat murine host cells when xenografted?

What do we know about the interaction of cancer cells with murine host cells? And how are murine host cells replaced by cancer cells?

09 October 2017 7,040 8 View

I constantly have floating cells in my SK-BR-3 culture. Is it normal?

Cells that adhered look healthy, but I am worried about the number of floating cells in my culture. Is it normal to have a significant number of floaters in SK-BR-3 culture? I am using RPMI 1640...

07 August 2016 10,067 5 View

Which statistical test is optimal for assessing drug synergy?

I came across several statistical tests to assess synergy between 2 drugs, among which are the Chou Talalay method and Bliss Independence model. Which one is optimal? And can the Chou Talalay...

07 August 2016 2,198 1 View

Does exercise reverse cachexia in cancer patients?

Food for thought.

07 August 2016 2,687 3 View

How much time does the freezing down media last?

For example, if it was made on the 27/3/2013?

03 April 2013 3,433 4 View

Anyone with a good plant extract protocol that ensures maximum efficiency in the extraction?

I'm working on a plant and checking its anti-proliferative activity.

03 April 2013 6,497 5 View

Best mounting buffer preparation?

There's a problem with my mounting buffer, it is always liquid and always tends to enter under the cover slips which interferes negatively while imaging ...

03 April 2013 9,273 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View