This for the study of the CEA protein in a model of colon carcinoma.
Does anyone know this larva? It was obtained from a dog's blood and analyzed using the modified Knott test to search for microfilariae. We think it might be contamination from the dog's fur as it...
14 November 2023 574 2 View
Hi everybody, I'm planning to sequence several phytoplasma strains by doing a nested PCR and cloning the resulting amplicons. Standard phytoplasma universal primers pairs will be used (P1/P7...
08 November 2020 2,631 4 View
Hi, I'm a plant pathologist (not a very good plant biologist I'm afraid) currently struggling. I was studying the spread of a disease on Manila palms (Adonidia merrillii) when I noticed some...
26 August 2019 2,632 8 View
Hi, I'm studying phytoplasma related-diseases on palms and for proper description of a syndrome recently detected, I need to identify this palm to the species level. If more photos are needed of...
24 July 2019 5,555 15 View
I want to introduce the gene of CRE-ERT2 in a murine cell line, but I do not know if is that possible.
08 September 2018 3,925 0 View
08 September 2018 340 0 View
After the insect feeds, do you have to threat the medium in any way (use an extraction protocol)? can you analyse it directly (using the medium as template)? I'll be using a real-time PCR protocol...
29 August 2017 8,241 1 View
What I need is a database of not (necessarily) published information. E.g., clinical side effects, measurement of quality of the environment factors from any nation, genetic stuff, etc. Any...
02 March 2016 6,373 4 View
When does this kind of memory begin? Can I talk about procedural memory in insects, for example?
19 March 2015 9,391 9 View
Each year, my undergraduate students are tested on their knowledge of general culture and science. I ask them about the age of the Earth, the beginning of known-life, the age of the modern humans...
01 January 1970 2,042 13 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....
10 August 2024 8,621 1 View
Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...
10 August 2024 8,992 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Who will bear moral responsibility for the deaths of thousands of people in the event of an earthquake? Weeks and months remain before the onset of strong earthquakes that bring death to...
08 August 2024 6,134 12 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View