I want to introduce the gene of CRE-ERT2 in a murine cell line, but I do not know if is that possible.
Does anyone know this larva? It was obtained from a dog's blood and analyzed using the modified Knott test to search for microfilariae. We think it might be contamination from the dog's fur as it...
14 November 2023 574 2 View
Hi everybody, I'm planning to sequence several phytoplasma strains by doing a nested PCR and cloning the resulting amplicons. Standard phytoplasma universal primers pairs will be used (P1/P7...
08 November 2020 2,631 4 View
Hi, I'm a plant pathologist (not a very good plant biologist I'm afraid) currently struggling. I was studying the spread of a disease on Manila palms (Adonidia merrillii) when I noticed some...
26 August 2019 2,632 8 View
Hi, I'm studying phytoplasma related-diseases on palms and for proper description of a syndrome recently detected, I need to identify this palm to the species level. If more photos are needed of...
24 July 2019 5,555 15 View
08 September 2018 340 0 View
After the insect feeds, do you have to threat the medium in any way (use an extraction protocol)? can you analyse it directly (using the medium as template)? I'll be using a real-time PCR protocol...
29 August 2017 8,241 1 View
What I need is a database of not (necessarily) published information. E.g., clinical side effects, measurement of quality of the environment factors from any nation, genetic stuff, etc. Any...
02 March 2016 6,373 4 View
When does this kind of memory begin? Can I talk about procedural memory in insects, for example?
19 March 2015 9,391 9 View
This for the study of the CEA protein in a model of colon carcinoma.
01 February 2013 5,925 0 View
Each year, my undergraduate students are tested on their knowledge of general culture and science. I ask them about the age of the Earth, the beginning of known-life, the age of the modern humans...
01 January 1970 2,042 13 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View