How to concentrate a PCR product before sequencing?

Hi everybody, I'm planning to sequence several phytoplasma strains by doing a nested PCR and cloning the resulting amplicons. Standard phytoplasma universal primers pairs will be used (P1/P7...

08 November 2020 2,155 4 View

What's the propper way to analise with PCR an artificial feeding medium (sucrose at 15%) for a planthopper for the presence of phytoplasma DNA?

After the insect feeds, do you have to threat the medium in any way (use an extraction protocol)? can you analyse it directly (using the medium as template)? I'll be using a real-time PCR protocol...

29 August 2017 8,126 1 View

What are the higher biomedical database freely accessible for researchers?

What I need is a database of not (necessarily) published information. E.g., clinical side effects, measurement of quality of the environment factors from any nation, genetic stuff, etc. Any...

02 March 2016 6,254 4 View

I am searching for the cell line MC38/CEA2, does anyone know who could send me this?

This for the study of the CEA protein in a model of colon carcinoma.

01 February 2013 5,844 0 View

Is it religion an obstacle for development of science in the undeveloped latin-american countries?

Each year, my undergraduate students are tested on their knowledge of general culture and science. I ask them about the age of the Earth, the beginning of known-life, the age of the modern humans...

01 January 1970 1,944 13 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View