I added too much isopropanol for DNA extraction for CLL(puregene method). The DNA I received was reddish. Will it degrade the DNA/affect the analysis?

More Susmita Augni's questions See All

How would I make hypercholesterolemia in Mice?

I am working on Mice and want to make hypercholesterolemia. But after giving cholesterol powder the level of cholesterol has decreased from previous level. Could anyone please suggest me how will...

26 February 2020 9,691 3 View

Why hasn't geopolymer based concrete been used that much?

OPC is the 2nd largest generator of CO2 after automobile industries. Researchers have claimed that geopolymer based concrete is more stronger , durable,versatile and eco friendly but...

04 March 2019 8,312 20 View

Which of the Clathrin Anitbody works well with murine cells?

Hello everyone, I have been using D3C6 from cell signaling and MA1-065 (monoclonal X22 from Thermofisher. I feel that they donot work well.Can you please tell me about other antibodies besides...

08 January 2017 3,616 4 View

Could Any one suggest for chromium estimation by UV Vis method using DPC?

Cr(vi) using DPC I mean what should be the ppm level to get a calibration curve. I tried but more than 10ppm the complex is very weak and gradually disappears. Any comment/suggestion is appreciated.

24 July 2016 1,559 3 View

What is the impact of climate change on indigenous people and isolated/ marginal communities in Mountains?

Climate change has impacted everywhere, every-sector and peoples.The most vulnerable group are mountain communities. Therefore it would be good to know about the case studies if available.

27 August 2012 7,281 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

Is is possible to pole with corona poling pellets of the sample material instead of thin films?

As it is highly probable that pellets will have a higher degree of air gaps and voids than thin films, is it possible to pole the samples with corona poling nevertheless? Or is there the...

24 February 2021 1,349 1 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View

Are there any known solvothermal methods to synthesize MoS2 nanosheets using solvents other than water (like ethanol/ethanol-water mixture) ?

I need to synthesize MoS2 nanosheets by alternate methods without pure water as a solvent.

22 February 2021 9,246 1 View

What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View

Can ethanol be used as an analgesic?

If ethanol has analgesic properties, why isn't it used for clinical purposes?

18 February 2021 8,227 4 View

What is the best way to isolate Hydrolysable tannins on Sephadex column?

I am currently preparing to isolate polyphenolics (Hydrolysable tannins) from aqueous extract of a plant. I want to set up a small sephadex column to guide the major isolation on a laerge...

18 February 2021 1,455 3 View