I added too much isopropanol for DNA extraction for CLL(puregene method). The DNA I received was reddish. Will it degrade the DNA/affect the analysis?

More Susmita Augni's questions See All

By using which software I can compare my XRD data with JCPDS ?

Dear Researchers, I need to compare my XRD data with JCPDS files and determine. please suggest me some software or some open database from where I can do this. I would greatly appreciate your help...

18 June 2023 8,266 2 View

Can you please help derive an equation for Rau with respect to the other parameters from this circuit?

Can you please help derive an equation for Rau with respect to the other parameters from this circuit? Thanks a lot!

27 April 2023 8,432 2 View

What might be the reason Pseudomonas pyocyanin production not evident on nutrient agar?

Pseudomonas aeruginosa is not showing pigmentation when incubated at 37 degree centrigrade for 48 hours on nutrient agar plate. Is any component of the media responsible for it?

24 October 2022 9,805 0 View

How can I use a plant growth incubator?

We have HANKUK S&I'S TWIN CHAMBER PLANT INCUBATOR in our lab, can anyone please help me to about the temperature setting process of the incubator?

04 August 2022 3,909 0 View

Regarding Abaqus output request?

Can we request acceleration as output in history output request in Abaqus/Cae V6.14-1 because I am not finding any option for acceleration in history output request.

20 April 2022 5,149 3 View

Regarding cyclic pushover analysis in Abaqus?

I am trying to model a concrete-filled steel tube pile in abaqus and currently I am trying to validate the model doing cyclic pushover analysis. I am applying interaction in step 1, fixed boundary...

10 March 2022 8,605 3 View

What is the status of 'Journal of Chengdu University of Technology'? Is it scopus?

http://cdujournal.com/

18 July 2021 5,949 10 View

What is the relevance of Hindu Marriage sacrament (Samskar) ?

Sacrament or Samskar means purification, Hindu have sixteen samskaras from Pre-birth to death. Marriage sacrament or Vivaha samskar is one of the sixteen samskaras.

29 June 2020 9,647 10 View

Is there any penal sanction for infringement of 'moral rights' or Author's Special Rights under the Copyright Act of India?

In India, under Section 57 of the Copyright Act, moral rights or author's special rights are: right to claim authorship and right to restrain or right to claim damages for distortion or mutilation...

15 March 2020 1,227 3 View

How would I make hypercholesterolemia in Mice?

I am working on Mice and want to make hypercholesterolemia. But after giving cholesterol powder the level of cholesterol has decreased from previous level. Could anyone please suggest me how will...

26 February 2020 9,759 3 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

How does grain and grain boundary affect the ceramic when studying its dielectric properties?

I am not able to get good literature and the physics behind how first these grains and grain boundaries arises out of no where when we make a pellet to study its dielectric properties and then how...

07 August 2024 5,177 3 View

How to increase protein lysate concentration?

I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...

01 August 2024 7,199 1 View

What is the best practice for resuspending cell pellets during competent cell preparation (both electro and comp)?

I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...

31 July 2024 8,309 0 View

For the moringa oleifera extract using ethanol as the solvent,what is the alternative method to concentrate the extract other than rotary evaporation?

99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified

31 July 2024 5,113 4 View

Comparison of Methanol and ethanol treatment on collagen based scaffolds?

Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you

24 July 2024 3,937 0 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

How to determine tannin content in fodder pellets?

22 July 2024 8,163 3 View

Why my plant protein cannot resolubilize after TCA/Acetone extraction?

I am extracting proteins from plant samples and am currently attempting to use the TCA/Acetone extraction method. I am having trouble resolubilizing my sample after drying the pellet. I collected...

15 July 2024 9,025 3 View

Do you know about fully Ethanol toyata Inova car?

https://youtu.be/5MckkqF9XWU?si=MvBsElJZg8_At8qP

09 July 2024 6,038 5 View