What can be the probable buffer composition to transfect in Jurkat cell line using electroporation?

I am trying to transfect my protein in Jurkat cell line but following a lot of issues. I am using 1X PBS as transfection agent, but the transfection level is low. Can anyone kindly suggest some...

29 July 2020 8,176 1 View

Problem is in running Gaussian 09 job (Windows). After submitted the job is immediately deleted. The check-route says 'file is not specified'. Why ?

I am using Gaussian 09 (windows). The problem has generated over last few days. Whenever I submit the job, it is immediately deleted with no error message and the output file is empty. The...

10 July 2020 2,604 1 View

How would I make hypercholesterolemia in Mice?

I am working on Mice and want to make hypercholesterolemia. But after giving cholesterol powder the level of cholesterol has decreased from previous level. Could anyone please suggest me how will...

26 February 2020 9,691 3 View

Why hasn't geopolymer based concrete been used that much?

OPC is the 2nd largest generator of CO2 after automobile industries. Researchers have claimed that geopolymer based concrete is more stronger , durable,versatile and eco friendly but...

04 March 2019 8,312 20 View

Which of the Clathrin Anitbody works well with murine cells?

Hello everyone, I have been using D3C6 from cell signaling and MA1-065 (monoclonal X22 from Thermofisher. I feel that they donot work well.Can you please tell me about other antibodies besides...

08 January 2017 3,616 4 View

Could Any one suggest for chromium estimation by UV Vis method using DPC?

Cr(vi) using DPC I mean what should be the ppm level to get a calibration curve. I tried but more than 10ppm the complex is very weak and gradually disappears. Any comment/suggestion is appreciated.

24 July 2016 1,559 3 View

Is the chemical synthesis of peptides necessary?

I am working on generating bioactive peptides from a natural source. I have read a few articles where people chemically synthesize the peptides after they have identified it in their bioactive...

10 March 2015 3,720 8 View

Can anyone recommend a pressure cell for transmission spectroscopy on polymer thin film under pressure?

I am in search of a pressure cell with optical (mostly visible wavelength regime) windows, where I can place a soft polymer thin film and squish it under controlled calibrated pressure for...

04 December 2012 7,150 9 View

What is the impact of climate change on indigenous people and isolated/ marginal communities in Mountains?

Climate change has impacted everywhere, every-sector and peoples.The most vulnerable group are mountain communities. Therefore it would be good to know about the case studies if available.

27 August 2012 7,281 3 View

Does it make sense to think of a completely empirical Physics dispensing altogether with the presiding Metaphysics ?

01 January 1970 7,367 60 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

How to model the battery pack in PSIM or PSCAD?

Hello, I have simulated a single battery cell using Thevenin equivalent circuit. For a battery pack, I have a large number of cells connected in series and parallel connections. Is there any way...

28 February 2021 2,116 1 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

How to design a boost converter for a mppt controller?

I have a doubt on how to select the inductor and capacitor value when I want to implement MPPT controller. This is because we always take a maximum output voltage and current but in this case, we...

28 February 2021 3,637 2 View

PMSM Inter Turn Short Circuit fault, Dataset?

Hi, I'm looking for a dataset of a PMSM with an inter turn short circuit fault. Does anyone know a dataset which is available?

21 February 2021 5,663 2 View

Why are the modelled and circuit system different in response for controlled buck converter?

[Images are attached at bottom] I am currently working on a buck converter controlled by two different control methods, Reinforcement learning applied to find PID values & a lead-lag...

16 February 2021 5,060 10 View

How to derive equations for a resonant buck converter with ZVS?

I am not understanding how each author is deriving the equations and the working principle. Individual authors are giving their own working principle explanation for different circuit topology....

12 February 2021 496 1 View

Is an Algal Microbial Fuel Cell a good research topic?

What do you think about studying the electricity production in an Algal Microbial Fuel Cell? Namely at the anode there is for example a solution with bacteria which degradate organic matter into...

27 January 2021 5,681 5 View

Can you tell the pros and cons of the proposed active AC coupling circuit?

Are there analog circuit designers? Here is a small task for you. The active circuit below aims to improve the AC coupling in the biosignal amplifiers. Is that so? Are there any pros? I'm...

17 January 2021 3,548 14 View

Use of Sine voltage source in Electrical circuit with frequency domain study in COMSOL?

Dear Sir/Madam I want to use Sine voltage as a source in an RLC circuit. I want to study the driving point impedance with frequency sweep. But when I use sine voltage, I get an error like The DAE...

13 January 2021 9,826 5 View