Hybridoma cell cultures with HT medium?

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Why my pcr bands are fuzzy?

Hi Although the ladder is sharp but my pcr bands are too fuzzy and like smears. I try to set up one of my genes but it still stands for 3 bands, unfortunately. Tm is 65 but there are still...

17 June 2024 7,412 1 View

Do we have to do all our cellular tests by treating with ic50 con?

Hi, I have done mtt test and realized that my reagent ic50 is 50nM. Do I have to do ally cellular assay by treating with this concentration? Do we do the mtt test for this purpose? Of course I...

10 May 2024 1,314 2 View

How to determine hydrogen peroxide concentration in a sample?

Actually as I searched about, there are two major method in determining hydrogen peroxide concentration, one is titration with sodium thiosulfate and other is titration with permangenate, I wanted...

29 April 2024 3,565 4 View

Where can I get a sinogram CT scan of the brain?

I am a graduate student writing to humbly request your assistance. I am currently working on my thesis which focuses on medical image reconstruction techniques. For my research, I need real...

27 September 2023 1,794 3 View

What is the best protocol of RNA( lncRNA) extraction using trizol reagent from plasma ?

I used this protocol but I did not answer 1) add 250 µl plasma to 750 µl Triazole 2) 5 min incubation 3) add 150 µl chloroform, mix 12 sec, 3 min incubation 4) Centrifuge at 10,000g for 10 min at...

11 August 2023 4,191 0 View

Why do I have smear in my PCR product loading on the gel (1%) for both the test samples and the water control (DW)?

I have set up a PCR using primers that amplify only the exons of my gene of interest. The template I am using is cDNA (mouse samples). I set up this PCR a year ago with an annealing temperature of...

30 July 2023 256 15 View

What is the wavelength of Glycerol monostearate maximum absorption in spectrophotometric analysis?

in order to quantify the concentration of Glycerol monostearate in aqueous solution by UV spectrophotometer, what is the maximum lambda of Glycerol monostearate?

13 May 2023 4,307 3 View

I have some questions about the culture of activated Platelet?

I work on Platelet and need to some information for culturing. How can activate platelet I mean which activator and how much time? as well as how can be stop this process without platelet...

08 May 2023 2,897 0 View

Image reconstruction code request‏‏‏‏?

Hello, I'm currently working on a project in lesion detection and I'm looking for image reconstruction codes for algorithms related to this field.(ASIR. 3DRP. FORE FBP. FORE OSEM. IMR or SAFIRE) I...

19 April 2023 2,712 2 View

Image reconstruction code request?‏‏‏‏

Hello, I'm currently working on a project in lesion detection and I'm looking for image reconstruction codes for algorithms related to this field.(ASIR. 3DRP. FORE FBP. FORE OSEM. IMR or SAFIRE) I...

19 April 2023 9,065 0 View

What are the long-term impacts of incarceration on youths' developing brain?

I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...

12 August 2024 862 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

What should Berlin do as a city to become as impactful as London and Paris in World Football?

Please go through my Abstract. I can also share a proposed Thesis Outline.

04 August 2024 2,077 0 View