Dear my colleages
I am culturing hybridoma cells about 3 weeks. Now They are in the T-flask.
I don't know how many times I must culture them in HT Medium?
What do you mean by "how many times"? How Long or how often you should change the medium?
I mean "How long is necessary to remove all of aminopterm from medium?".
hello.afer cloning you can use HTmedium but before cloning you can use HAT medium.
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