What are some good polymer to use for packaging a biological product ,which can be steril by gamma?

As different polymers with different characteristics are exist,I want to know What polymers can be used for packaging an orthobiologic product(bone allograft),which can have enough resistent to...

22 December 2020 8,196 5 View

For LDH activity, is there any difference to add NADH, pyruvate and Tris separately in the well or prepare a mixture of them and then add?

Hi everybody, For LDH enzyme activity, I prepared to different series of buffers based on two protocols: 1-I prepared the solutions of 100 mM Tris, 3.6 mM Sodium pyruvate, 3.4 mM NADH...

22 October 2020 9,855 3 View

Nursing article about corona virus?

Hello Dear I need to catch scholarly articles about people attitude about contagious diseases like corona virus and how to mange their fear despite of maintaining their sensation about it

23 March 2020 6,936 12 View

Thin films of tio2 is made with sol gel (dip-coating) I want to know why when thin film thickness of Tio2 increases, anatase change to brookite?

Thin films of TiO2 is made with sol gel (dip-coating) I want to know why when thin film thickness of TiO2 increases, anatase change to brookite?

26 April 2018 2,142 4 View

Can anybody help me about removing of endotoxin from bacteriophage?

Hi. How can I remove endotoxin from bacteriophage lysate with a simple and high-efficiency method?I will appreciate to help me.

17 October 2016 4,544 1 View

How can I get the clear and high-quality image of my cells have been transformed by GFP vector under florescent microscope?

06 September 2016 6,924 4 View

When results of otsu method is better?for more levels or lower levels?

17 June 2016 1,571 3 View

Hybridoma cell cultures with HT medium?

Dear my colleages I am culturing hybridoma cells about 3 weeks. Now They are in the T-flask. I don't know how many times I must culture them in HT Medium?

27 February 2016 5,245 3 View

Which thresholding techniques are better if our image has Intensity homogeneities?

27 October 2015 9,872 17 View

I am looking for SAS software code for Augmented Design. Can someone help me out?

30 January 2015 2,107 18 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

HPLC baseline failure (ACN/Water)?

My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...

21 February 2021 5,656 3 View