I work on Platelet and need to some information for culturing.

How can activate platelet I mean which activator and how much time? as well as how can be stop this process without platelet damaging, without degranulation, following that I want to use this activated platelet for co-culture.

-Is it possible I use the activator in the medium of co-culture, I mean if the activator stay in medium for 24h and 48h, whether platelet will be lysed?

- is there any way for activating platelet without lysing?

- whether platelets need to stir mild in co-culture time or not?