6 Questions 12 Answers 0 Followers
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My colleagues, I want to remove embryo from mice in many days of pregnancy period,now I have some question: 1.Is there a method for exact detection of days in pregnancy period except of seeing...
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Dear my colleages I am culturing hybridoma cells about 3 weeks. Now They are in the T-flask. I don't know how many times I must culture them in HT Medium?
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I am growing cells in DMEM+FBS10% , but most of cells died after passaging. can someone help me?
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Which medium is better for monoclonal antibody production in hybridoma protocol, DMEM or RPMI-1640? Why? and what are the component of each media? Thanks.
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Especially in hybridoma protocol.... I fused cells but I can not diagnose hybridoma cells from myeloma and spleen cell. Thanks
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I want to do IP injection to mouse. I tend to inject 50 microgram of protein but I don't know in 1ml of drug how many gr of protein there are?
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