Atefeh Hemati

6 Questions 12 Answers 0 Followers

Questions related from Atefeh Hemati

What is the process of mice embryo fixation?

My colleagues, I want to remove embryo from mice in many days of pregnancy period,now I have some question: 1.Is there a method for exact detection of days in pregnancy period except of seeing...

06 October 2016 4,815 7 View

Hybridoma cell cultures with HT medium?

Dear my colleages I am culturing hybridoma cells about 3 weeks. Now They are in the T-flask. I don't know how many times I must culture them in HT Medium?

28 February 2016 5,378 3 View

What is the best way for growing sp2/0 cells?

I am growing cells in DMEM+FBS10% , but most of cells died after passaging. can someone help me?

30 August 2015 1,509 4 View

What is a good medium for monoclonal antibody production?

Which medium is better for monoclonal antibody production in hybridoma protocol, DMEM or RPMI-1640? Why? and what are the component of each media? Thanks.

04 August 2015 3,615 6 View

How can I dignose dead cells and living cells in cell culture?

Especially in hybridoma protocol.... I fused cells but I can not diagnose hybridoma cells from myeloma and spleen cell. Thanks

11 June 2015 4,589 7 View

IP injection dosage?

I want to do IP injection to mouse. I tend to inject 50 microgram of protein but I don't know in 1ml of drug how many gr of protein there are?

16 August 2014 3,896 1 View