in order to quantify the concentration of Glycerol monostearate in aqueous solution by UV spectrophotometer, what is the maximum lambda of Glycerol monostearate?
Dear friend Atefeh Shiri
The wavelength of maximum absorption for glycerol monostearate can vary depending on the solvent used and the concentration of the solution. However, a study published in the Journal of Lipid Research found that the maximum absorption wavelength for glycerol monostearate in methanol is around 230-235 nm. (Reference: https://www.jlr.org/content/16/6/674.abstract)
It is important to note that the specific wavelength of maximum absorption may vary depending on the instrument used and the experimental conditions, so it is recommended to conduct a calibration curve using different concentrations of glycerol monostearate and measuring the absorbance at different wavelengths in order to determine the most suitable wavelength for quantification.
Dear Kaushik Shandilya,
Thanks for the helpful and detailed answer.
Could you please share the article doi too?
Glycerol monostearate (GMS) does not have a specific wavelength of maximum absorption in spectrophotometric analysis because its absorption characteristics can vary depending on various factors such as concentration, solvent, and experimental conditions.
In general, the absorption spectrum of GMS typically shows absorbance peaks in the ultraviolet (UV) or visible range. To determine the wavelength of maximum absorption for a particular sample of GMS, it is necessary to measure its absorption spectrum using a spectrophotometer.
If you have a specific GMS sample and need to determine its absorption characteristics, you can perform a UV-Vis (ultraviolet-visible) spectroscopy experiment. By scanning the sample across a range of wavelengths (typically from 200 nm to 800 nm), you can observe the absorption spectrum and identify the wavelength(s) of maximum absorption. This information can then be used for further spectrophotometric analysis of GMS
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