ECL versus CLIA?

Hi everybody. I am interested to know what is different between: electrochemiluminescence (ECL) method and chemiluminescent immunoassay (CLIA) method ? TNX... Kambbiz

11 December 2018 1,646 4 View

What Proteome Information should hunt after from core seminal plasma proteome?

Dear all I have mapped the proteome of human seminal plasma. Furthermore, I have compare it with the plasma proteome. I want to know what information I can extract from my core seminal plasma...

09 October 2018 3,766 2 View

Analytical or Preparative 2DE gel for image analysis?

Hi Everyone We are running 2DE in our lab. I am in doubt what is best for running image analysis: It is best to run a analytical or preparative 2DE gel for image analysis between control or...

08 September 2018 3,460 0 View

FDA approved metabolite identification by HPLC or LCMS?

Hi Everybody I am looking for a paper or the list of FDa approved metabolite identified by HPLC or LCMS? Can you help me out? Merci, Kambiz

05 June 2018 8,692 7 View

What is the number of all possible metabolic reactions?

Hi Everybody. I am looking for a paper or database about the number of all possible metabolic reaction in different organisms e.g. human or E.coli! Merci

31 December 2016 8,447 2 View

How many genome have been sequenced so far?

Hi Everybody. I am looking for documentation about how many genome have been sequenced so far. Additionally, how many gene are found from these organism? Merci, Kambiz

31 December 2016 9,085 0 View

What tense to use to write a good review paper?

Hi Everybody I want to write a good review based on my results and the literature. However, I do not know which tense I should use? Is it dependent on journal I have choose? Merci, Kambiz

09 October 2016 722 6 View

Plasma proteome vs seminal plasma proteome. How can I do it in a smart way?

Hi Everybody. I have collected more than 2000 proteins from human seminal plasma. I want to compare it to the human plasma proteome. Is there any smart software out there which can help me...

05 June 2016 3,406 2 View

How to define Systems Proteomics?

Hi Fellows. I was wondering how to define Systems Proteomics? Does anybody have a good suggestion. I have attache those paper I could find regarding this approach... Merci, Kambiz

05 June 2016 7,056 5 View

How many 2DE gels run for clinical proteomics trial?

Hej Everybody. I am looking for to get answer how many 2DE gels I have to run for a pilot study in clinical proteomics? Should I pooled the sample and run three 2DE-gels or just run 3 good...

04 May 2016 6,630 4 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

What is the best method to evaluate and measure non-circulating estrogen levels in mice ?

Could anyone suggest me the best procedure to measure the circulating estrogen levels in mice ? I came across the general methods where it can be measured in serum or urine but a detail suggestion...

22 February 2021 4,352 2 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View