Hej Everybody. I am looking for to get answer how many 2DE gels I have to run for a pilot study in clinical proteomics? Should I pooled the sample and run three 2DE-gels or just run 3 good 2DE-gels and run western-blot on the potential biomarker? How high the fold changes should be for quantification to be real? I read different paper and some reported more than 1.5 fold change etc. The best value is not 2 fold changes?

Merci,

Kambiz

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