Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

How to generate .dlg files on PyRx?

Hi. I'm using the PyRx version 0.8 and getting the error message: WARNING: Filename mismatch: "2DFN_ADP_CL.maps.fld" :: "..\2DFN_ADP_CL.maps.fld" C:\Program Files (x86)\The Scripps Research...

17 January 2021 843 3 View

Confirming what statistics I need to run for a pilot study in social sciences?

I have altered two instruments and collected a sample of n=60. 1. For the first instrument, I changed the wording of the items to refer to life instead of work. I believe checking the cronbach...

13 March 2020 7,391 3 View

Recovery rate for PD-10 desalting column: Any idea?

Hi all, I have high levels of protein loss on PD-10 desalting column (gavity flow desalting column) when trying to buffer exchange. According to GE, the recovery rate should be higher that 70%,...

03 October 2019 8,483 3 View

Why did I get RMSD plot with 10ns if the MD was 100ns trajectory?

I am new in gromacs and I'm experiencing some difficulties in the analysis of my results. I calculated the RMSD of backbone and ligand from the MD with a trajectory of 100ns (nsteps = 50000000),...

04 September 2019 3,669 6 View

Reversed Flow in Spillway Simulation in Fluent?

Hi I'm trying to simulate the flow of water over an ogee spillway in Fluent. My problem is that I'm getting reversed flow on my pressure outlets. I am aware that it might not affect significantly...

05 December 2018 6,108 3 View

How can I quantify exosomes protein from cell culture media?

I've isolated exosomes from 5ml of cell culture media using ExoQuick TC PLUS kit. I got a visible pellet that was resuspended in the "resuspension buffer" and then purified with the...

13 June 2018 5,514 4 View

What method is best or standard to determine Glut1 expression in T cells?

I am trying to decide the best method between flow cytometry or qPCR to be used as I compare Glut1 expression in two different T cell lines. Is there an ideal protocol? I had read articles where...

17 March 2018 4,211 3 View

How to Build a silica nanosphere with Materials Studio?

Hello, I am beginner in Materials studio software and also in Molecular dynamics and I am needing to build a silica nanosphere with different numbers of hydroxyl groups to adsorb some molecules....

27 December 2017 5,577 2 View

How to preserve cells for FISH?

Hello! I am going to do FISH and I have to make my experiment soon, but the probes are not here yet. How can I better store the cells and for how long? I saw in one protocol that you can leave in...

26 June 2017 7,386 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

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02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View