I need a certain method to cause irreversible DNA denaturation in double stranded FIsh DNA?

I need a certain method to cause irreversible DNA denaturation in double stranded FIsh DNA

01 February 2019 6,717 3 View

Dose equivalent to niosome encapsulation dose?

I'm preparing niosome encapsulating a certain natural extract how can I caclulate the encapsulated dose which is equivalent to the free dose of that substance?

11 December 2018 7,655 3 View

Animal dosage from mgs to mls?

I want to treat rats with a certain drug loaded on a nanocarrier with a certain dose... The problem the i have.. That when converting my formulation to powder through air drying.. The yield is...

01 February 2018 7,203 3 View

Total proteins extraction?

I want a simple protocol ( non-costly) for the separation of total proteins from breast tissues without altering their conformations

01 February 2018 5,784 1 View

Total proteins extraction?

Would someone suggest a simple protocol for total proteins extraction from human tissues that don't alter protein conformation ?

01 February 2018 3,006 2 View

Best solvent for Thymoquinone?

What is the best solvent for IP injection in rats for TQ for one week treatment ?

01 February 2018 1,059 1 View

Best solvent for chitosan nanoparticles ?

what is the best solvent for chitosan nanoparticles for IP injection in rats for one week treatment ?

01 February 2018 7,361 2 View

DNA extraction protocol ?

Would someone tell me a successful simple protocol for DNA isolation from animal tissues , that gives reasonable yield and which is not costly and does not alter DNA structure ?

01 February 2018 8,545 7 View

Cel membrane isolation?

What is the simple organism or cell type from which I can isolate its membrane and what is the best model to study the interaction of nanoparticles with cell membrane ?

31 December 2017 4,319 3 View

Encapsulated drug dose calculation?

I will make an in vivo experiment in which I want to compare between the effect of a free drug and the same drug loaded on chitosan nanoparticles.. how can I calculate the dose of nanoparticles...

31 December 2017 4,358 2 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

No title

22 February 2021 9,932 11 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

SPSS: A scale with multiple items (7-point Likert) across 4 test conditions. How can I correctly summarize it?

Hello, I am currently writing my master thesis where I have a 2x2 factorial design with one downstream variable (2 independent and 2 dependent variables). I need to conduct a multivariate...

11 February 2021 6,014 4 View

Why stop the reaction in ice for the fluorescent probe FDA (fluorescein diacetate)?

Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...

08 February 2021 3,366 3 View

What is the possible cause of MSCs low growing?

Hello Everyone, We are inexperienced in culture of MSCs trying to grow a sample from BM of a mare. It is the second time that after preparation of new medium (DMEM-F12 with high glucose - 10% Hy...

07 February 2021 9,989 7 View