I want to get some images of how nanoparticles work in bacteria cells. I used scanning electron microscopy. how to make samples, and how much use bacteria concentrations?
Dear Ashu Devraj
To prepare samples for SEM imaging of bacterial cells treated with nanoparticles, you can follow the below steps:
As for the bacteria concentration, it depends on the type of bacteria you are using, the nanoparticle concentration, and the experiment conditions. However, generally, a concentration of 10^8 to 10^9 bacteria per ml is often used for SEM imaging.
If you want to see whether nanoparticals penetrate into bacteria, you need TEM, not SEM. If you want to see changes in biofilm after treatment, use protocol from previous answer by Kaushik Shandilya
Try the protocol mentioned in following publication
Article Nanomaterials for the control of bacterial blight disease in...
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