We have prepared a Trolox solution of 1mg/ml. From this 2 fold dilutions were prepared up to 31.25ug/ml. we have mixed 700ul of Trolox from each dilution with 700ul of 20ppm DPPH. from this 200ul was loaded on to an ELISA plate.
Two controls were added in separate wells, one was absolute methanol and other distilled water as instructed. Now there are two absorbances for two controls. This makes it difficult to plot the Trolox calibration curve.
What can we do? Can we add the two absorbances of the two controls? Otherwise the calculated % scavenging activity would become negative.
We don't have the option of repeating the practical.
You should use as control the same solvent that you have used to prepare your analytical curve of trolox.
The solutions you have used are not control but blank. Your control must contain only DPPH solution without any compounds such as TROLOX.
Generally, your blank is the solvent used in the preparation of Trolox solution. However, as you have used the same volume of both TROLOX and DPPH solutions (700 ul), here you should use the same ratio of the solvents used in the preparation of these two molecules.
- Badges
- Science topic
- Similar topics
- Mathematical Sciences
- Graphs