Does anyone know why SDS-PAGE pattern of alpha-synuclein is different in different studies?

SDS-page pattern of alpha-synuclein in some studies appears to be higher (around 17 KD) than its real place of its molecular weight (14.4 KD). However, our purified protein appears at around 15...

18 July 2014 3,098 5 View

What is different between water & steam distillation and steam distillation system?

Attention please: we can use fresh materials in both system. I mean that we use fresh material

21 May 2013 6,034 5 View

Can I find a plant which contains Dicyclomine? Or any other plants with anti-chollinergic properties?

Dicyclomine is a compound with anti-cholinergic properties.

06 December 2012 627 6 View

Why do the absorbance values of different concentrations of ascorbic acid solutions mixed with DPPH free radicals stay constant?

26 January 2021 458 14 View

IC50 value from this graph?

01 January 2021 6,848 4 View

Is rotary evaporator a must before going through TPC, TFC and DPPH assay?

I am doing an optimization of ginger extract using doe software, firstly I extract the ground ginger with 20ml solvents of 60%, 70% and 80% ethanol using the ultrasound water bath in different...

23 October 2020 3,268 10 View

How to calculate IC50 of plant extracts dissolved in DMSO?

Greeting! Currently I am working with my plant extracts with different concentration on DPPH assay and anti-tyrosinase assay. All of my plant extracts with different concentration are dissolved...

02 October 2020 3,185 3 View

Why is it necessary to vortex and incubate in dark the sample and DPPH solution and other DPPH questions?

Hi all, In the determination of Antioxidant capacity of fruit samples, I used DPPH assay and have a few questions regarding the DPPH assay 1) why is it necessary to vortex and incubate in dark...

30 August 2020 246 3 View

How do I perform the correlation between phenolic contents of extractives with hydroxyl radical scavenging activity?

I want to show the correlation between total phenol and flavonoid contents of extractives with DPPH, hydroxyl, and ferric reducing scavenging activity. I would prefer to use GraphPad prism. Could...

25 August 2020 7,261 3 View

Why am I getting negative values for antioxidant assay standard curve?

I am trying to get a standard graph for antioxidant activity of ascorbic acid using 0.1 mM DPPH solution. I had used 100 ug/ml stock and serial dilutions were from 1 ug/ml to 10 ug/ml. All of the...

07 August 2020 1,835 3 View

What Solvent is appropriate for DPPH when extracting with ethanol?

I am working with seaweeds. I am using using ethanol as my extraction solvent based on preliminary experiment and using methanol to dissolve my DPPH, should I expect any difference than, when I...

09 July 2020 1,052 14 View

Why there is no color conversion of purple color to yellow while working with bitter gourd fruits dry powder by DPPH assay??

Why there is no color conversion of purple color to yellow while working with bitter gourd fruits dry powder by DPPH assay??

20 May 2020 6,010 5 View

Which Concentrations of Trolox and sample extracts should I use in my calculations for IC 50, DPPH assay?

I have a question about the DPPH assay. Recently, I have been busy with literature and several preliminary experiments to be able to choose the best conditions (extraction method, solvents) to...

11 March 2020 6,776 6 View