How to get rid of polysaccharides during RNA extraction?

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My cell line U87MG is getting detached after splitting, what could be the reason?

Few weeks back there was fungal infection in some of my petri dishes. So, I thoroughly cleaned the incubator using bleach and rectified alcohol, revived fresh stocks, prepared everything fresh and...

07 August 2015 2,754 17 View

How do we calculate scale for microscopic images and how do we add a scale bar to it?

I need to put scale bar in my microscopic images at 100x, 40x, 20x and 10x

04 May 2015 9,576 1 View

Can anyone help me please in calculating the IC50 dose of the drug using the Graph Prism software?

I have followed the tutorial still it is not helpful! please help

02 March 2015 7,056 2 View

Can we perform micronucleus assay without using cytochalasin B?

Since it is a very hazardous chemical I do not want to use it and also it is expensive too.

06 July 2014 1,445 17 View

please anyone give me a good and sensitive protocol for DNA fragmentation to check apoptosis in cancer cell lines

I am not able to repeat results with the previous protocol

04 May 2014 2,471 5 View

Can we perform flow cytometry on -80 frozen samples?

We collected the plants 2 months back and stored them in -80

03 April 2014 9,172 5 View

Under which filter should I view acridine orange and Etbr staining for checking cell viability of cancer cells?

I just want to know the cells should be viewed under which filter.

03 April 2014 706 2 View

I require caspase antibodies, can anyone help?

I require the caspases for western blotting.

02 March 2014 9,581 4 View

Can anyone suggest me a good lab in New Delhi, India where I can perform western blotting and flow cytometry of cancer cells after drug treatment?

I am really frustrated as I cannot perform it in my lab.

01 February 2014 2,229 2 View

How to design primers for EST-SSR Markers?

For differentiating between male and female species of a plant.

01 February 2014 7,243 5 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

Shailendra Dwivedi

yes ,during plant mRNA EXTRACTION, this type of problems may exist,please go through the attached paper,it may help.

Amit Kumar

you may try adding adding PVP in buffer... if your starting material is plant tissue.. try CTAB buffer folowed by isopropanol step.

Himanshi Kapoor

Thanks..