Himanshi Kapoor

43 Questions 95 Answers 0 Followers

Questions related from Himanshi Kapoor

My cell line U87MG is getting detached after splitting, what could be the reason?

Few weeks back there was fungal infection in some of my petri dishes. So, I thoroughly cleaned the incubator using bleach and rectified alcohol, revived fresh stocks, prepared everything fresh and...

08 August 2015 2,703 17 View

How do we calculate scale for microscopic images and how do we add a scale bar to it?

I need to put scale bar in my microscopic images at 100x, 40x, 20x and 10x

05 May 2015 9,517 1 View

Can anyone help me please in calculating the IC50 dose of the drug using the Graph Prism software?

I have followed the tutorial still it is not helpful! please help

03 March 2015 7,009 2 View

Can we perform micronucleus assay without using cytochalasin B?

Since it is a very hazardous chemical I do not want to use it and also it is expensive too.

07 July 2014 1,400 17 View

please anyone give me a good and sensitive protocol for DNA fragmentation to check apoptosis in cancer cell lines

I am not able to repeat results with the previous protocol

05 May 2014 2,436 5 View

Can we perform flow cytometry on -80 frozen samples?

We collected the plants 2 months back and stored them in -80

04 April 2014 9,120 5 View

Under which filter should I view acridine orange and Etbr staining for checking cell viability of cancer cells?

I just want to know the cells should be viewed under which filter.

04 April 2014 669 2 View

I require caspase antibodies, can anyone help?

I require the caspases for western blotting.

03 March 2014 9,533 4 View

Can anyone suggest me a good lab in New Delhi, India where I can perform western blotting and flow cytometry of cancer cells after drug treatment?

I am really frustrated as I cannot perform it in my lab.

02 February 2014 2,189 2 View

How to design primers for EST-SSR Markers?

For differentiating between male and female species of a plant.

02 February 2014 7,149 5 View

Any suggestions for getting smaller fragments of only 200bp range in DNA fragmentation, when earlier I was getting a range of fragments?

I was getting good results before, but all of a sudden when I repeated the procedure with the same cell line, same drug, and same drug concentrations, it went wrong. Please find attached before...

01 January 2014 6,798 5 View

In what ratio should i dilute my antibodies so as to develop the western blot?

I am short of antibodies, please tell me the minimum amount of undiluted antibodies required for conducting my experiment.

01 January 2014 7,735 9 View

Is DEPC treatment of glassware and plasticware necessary for RNA extraction?

Or normal autoclaving will do?

01 January 2014 6,385 13 View

Should we make fresh drug every time when we treat cancer cell lines?

Or will previously made drug do?

01 January 2014 6,889 3 View

What is a protocol for isolation of RNA from cancer cell lines manually?

Need it for semi quantitative expression of DR5.

01 January 2014 8,601 7 View

Which material is used to make the mesh in columns provided for gel elution?

It is this mesh, to which the nucleic acid binds

01 January 2014 4,596 1 View

How to discard etbr gels?

Is there any chemical methods to treat etbr gels , so as to precipitate etbr or negate its harmful effect

01 January 2014 9,188 3 View

What assays should I further perform to check and validate the effect of my drug on cell lines?

I have already performed MTT assay, Trypan blue exclusion and DNA fragmentation.

12 December 2013 6,061 7 View

Protocol for isolation of proteins from cancer cell lines?

It would be used for western blotting.

10 October 2013 2,603 8 View

What is the protocol for western blotting when looking for the expression of proteins of the apoptotic pathway?

The protocol is performed after drug treatment on the cancer cell lines. Please provide necessary details and intricacies, as I am new to this technique.

10 October 2013 3,667 3 View

How does good quality cDNA appear on a gel?

I am unable to amplify the band of my interest from the new cDNA, whereas the old cDNA used to give a very prominent band at 60 degrees. I have repeated the reaction umpteen number of times, with...

10 October 2013 3,340 6 View

DNA fragmentation protocol - can anyone help?

Without using kit.

09 September 2013 6,830 8 View

Why are my cryo-stocks of cell lines not reviving?

09 September 2013 1,580 24 View

For MTT assay how many cells should we seed per well?

I have been seeding 1000 cells per well.

09 September 2013 2,641 7 View

is it advisable to put sodium azide in the water which is put in the CO2 incubator for humidity

What conc. Should we add

09 September 2013 4,156 11 View

How do we do FACS based cell sorting?

What are its applications? Need detailed protocols.

09 September 2013 9,492 7 View

Apart from ATCC from where can I get authentic cell lines?

Is there any basis for authenticity?

09 September 2013 7,610 6 View

Can microbes travel across parafilm?

I kept my cultures in BOD and all of a sudden after 3 days of transfer they were contaminated. The first two days they were normal without any infection.

09 September 2013 1,110 5 View

In MTT assay after drug treatment do we incubate the cells in fresh media and then check the viability with MTT?

Or do we directly add MTT in the drug treated wells?

08 August 2013 6,960 25 View

How to get rid of polysaccharides during RNA extraction?

How can we get rid of wax?

08 August 2013 3,207 3 View

A protocol for DNA extraction from cancer lines?

The cell line is of neurological origin.

08 August 2013 4,580 26 View

Can someone help me regarding myo-inositol not dissolving in acetonitrile?

It is required for HPLC standard preparation.

07 July 2013 5,110 5 View

How to make a gene cassette (with promoter, terminator, marker gene and gene of interest)?

Are there any kits available for it?

04 April 2013 704 14 View

Can Inoue transformation buffer be autoclaved?

(I need it for making competent cells)

03 March 2013 9,681 3 View

Can anyone give me an efficient protocol for making competent cells of e.coli (DH5alpha) for heat shock and electroporation?

03 March 2013 3,040 12 View

What is the difference between tris-Hcl and tris base?

Is their buffering capacity different?

03 March 2013 2,314 27 View

I am going to design a real time PCR experiment, what things should I keep in mind?

What are necessary requirements, details, technicalities, precautions etc.?

03 March 2013 8,449 28 View

Do we dry our glassware in the oven after DEPC treatment or directly autoclave and then bake it in oven?

Will it affect RNA quality?

03 March 2013 3,095 7 View

what is the reason for obtaining water in the LB Agar plates with Ampicillin, IPTG, X-gal

I inoculated transformed E.coli on these plates, after electroporation for blue white selection.

03 March 2013 5,373 4 View

How to design degenerate primers?

To characterize a known gene in another plant system, in which it is not reported.

02 February 2013 7,303 17 View

Where can I get LdP line software from?

(For survival tests and probit analysis)

02 February 2013 6,507 8 View

Hot Start Taq Vs. Fusion Taq?

Which one should I rely on for cloning experiments?

02 February 2013 8,698 5 View

Please tell me which column to use for better resolution:- 1cm in diameter or 4cm in diameter.

The yield after running soxhlet is approx. 5g

02 February 2012 9,777 13 View