My cell line U87MG is getting detached after splitting, what could be the reason?

Few weeks back there was fungal infection in some of my petri dishes. So, I thoroughly cleaned the incubator using bleach and rectified alcohol, revived fresh stocks, prepared everything fresh and...

07 August 2015 2,580 17 View

How do we calculate scale for microscopic images and how do we add a scale bar to it?

I need to put scale bar in my microscopic images at 100x, 40x, 20x and 10x

04 May 2015 9,390 1 View

Can we perform micronucleus assay without using cytochalasin B?

Since it is a very hazardous chemical I do not want to use it and also it is expensive too.

06 July 2014 1,282 17 View

please anyone give me a good and sensitive protocol for DNA fragmentation to check apoptosis in cancer cell lines

I am not able to repeat results with the previous protocol

04 May 2014 2,341 5 View

Can we perform flow cytometry on -80 frozen samples?

We collected the plants 2 months back and stored them in -80

03 April 2014 9,013 5 View

Under which filter should I view acridine orange and Etbr staining for checking cell viability of cancer cells?

I just want to know the cells should be viewed under which filter.

03 April 2014 576 2 View

I require caspase antibodies, can anyone help?

I require the caspases for western blotting.

02 March 2014 9,439 4 View

Can anyone suggest me a good lab in New Delhi, India where I can perform western blotting and flow cytometry of cancer cells after drug treatment?

I am really frustrated as I cannot perform it in my lab.

01 February 2014 2,089 2 View

How to design primers for EST-SSR Markers?

For differentiating between male and female species of a plant.

01 February 2014 7,055 5 View

How to discard etbr gels?

Is there any chemical methods to treat etbr gels , so as to precipitate etbr or negate its harmful effect

31 December 2013 9,109 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

What is the most prevalent drug related problem in the developed countries?

Detecting the drug related problems is an important step in the pharmaceutical care plan

21 February 2021 5,421 3 View

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19 February 2021 8,647 3 View

How to improve dissolution of neosome?

I have made neosome from herbal drug. Neosomes are stable. But I face problems in dissolution. I used minimum ratio of 1:1:0.25 ( Drug:span 60: Cholesterol and GMs. Now how can I active better...

17 February 2021 3,584 1 View

How to determine the drug loading and the drug release capacity of a polymer micelle using UV spectroscopy?

Currently, I am working on PLA-PEG copolymer micelle for the drug delivery system. I have seen several papers where some researcher talks to centrifuge the drug-loaded micelle to separate the...

13 February 2021 5,822 7 View